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Biosynthesis, production and structu...

Vulpanovici, Florina Alexandra.

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Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria./
Author:	Vulpanovici, Florina Alexandra.
Description:	149 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6079.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116165
ISBN:	0496636014

Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria.
Vulpanovici, Florina Alexandra.

Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria. - 149 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6079.

Thesis (Ph.D.)--Oregon State University, 2004.

This thesis details investigations of marine cyanobacterial secondary metabolism, with emphasis on a strain of Phormidium sp. collected in Indonesia. These studies assessed the effects of nineteen putative elicitor compounds on the growth and metabolite production of five species of marine cyanobacteria, biosynthetic investigation of an intriguing secondary metabolite, phormidolide, and the discovery of one novel halogenated peptide, phormidamide.

ISBN: 0496636014Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria.
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020 $a 0496636014
035 $a (UnM)AAI3116165
035 $a AAI3116165
040 $a UnM $c UnM
100 1 $a Vulpanovici, Florina Alexandra. $3 1931637
245 1 0 $a Biosynthesis, production and structural studies of secondary metabolites in cultured marine cyanobacteria.
300 $a 149 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 6079.
500 $a Adviser: William H. Gerwick.
502 $a Thesis (Ph.D.)--Oregon State University, 2004.
520 $a This thesis details investigations of marine cyanobacterial secondary metabolism, with emphasis on a strain of Phormidium sp. collected in Indonesia. These studies assessed the effects of nineteen putative elicitor compounds on the growth and metabolite production of five species of marine cyanobacteria, biosynthetic investigation of an intriguing secondary metabolite, phormidolide, and the discovery of one novel halogenated peptide, phormidamide.
520 $a The growth, biomass production and the ratio of the components of the extract were affected by some of the elicitors in most of the cyanobacterial species tested. However, production of a novel secondary metabolite or a significant change in the bioactivity of the extracts was not observed.
520 $a Biosynthetic investigations of a brominated brine shrimp toxic polyketide, phormidolide, were conducted on a cultured Phormidium sp. strain originally isolated from Indonesia. Stable isotope feeding experiments confirmed its polyketide nature and established a new example of a general trend in cyanobacterial metabolism where both S-adenosyl methionine and C2 of acetate contribute to the biogenesis of pendant methyl groups. At the same time, feedings with deuterated acetate provided insight into the HMG-CoA synthase-like mechanism by which addition of pendant methyl groups from C2 of acetate takes place.
520 $a Studies of phormidolide production in bromine-depleted medium showed that two analogs are produced, debromophormidolide with a terminal olefin in place of the vinyl bromide, and iodophormidolide, introducing iodine in place of bromine from the trace amounts present in the medium. Supplementation of the bromine-depleted culture medium with iodine resulted in a 10-fold increase of iodophormidolide production, while bromine supplementation resulted in a more moderate (2.5 fold) enhancement in phormidolide yield.
520 $a A novel halogenated cytotoxic peptide, phormidamide, was isolated and a planar structure is proposed, pending confirmation by X-ray crystallographic analysis. Phormidamide contains a unique bromophenylalanine functionality, three chlorine atoms, and a very high number of quaternary carbon atoms which have hindered structural elucidation efforts through spectroscopic methods.
590 $a School code: 0172.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Pharmaceutical. $3 550957
690 $a 0487
690 $a 0491
710 2 0 $a Oregon State University. $3 625720
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Gerwick, William H., $e advisor
790 $a 0172
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116165