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The use of plant oilbodies as an ant...

Alcantara, Joenel.

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The use of plant oilbodies as an antigen production and delivery system.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The use of plant oilbodies as an antigen production and delivery system./
Author:	Alcantara, Joenel.
Description:	284 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0009.
Contained By:	Dissertation Abstracts International65-01B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ87013
ISBN:	0612870138

The use of plant oilbodies as an antigen production and delivery system.
Alcantara, Joenel.

The use of plant oilbodies as an antigen production and delivery system. - 284 p.

Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0009.

Thesis (Ph.D.)--University of Calgary (Canada), 2003.

In the early 1990's, the Children's Vaccine Initiative was initiated to develop new technologies that would make vaccines cost-effective in both production and delivery. One interesting possibility offered by recent advances in immunology, and plant biotechnology is the oral delivery of subunit vaccines in the edible parts of a plant. Before edible vaccines become a practical approach in combating diseases, however, several concerns will have to be addressed including variability in antigen expression and the induction of oral tolerance.

ISBN: 0612870138Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

The use of plant oilbodies as an antigen production and delivery system.
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100 1 $a Alcantara, Joenel. $3 1929095
245 1 4 $a The use of plant oilbodies as an antigen production and delivery system.
300 $a 284 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0009.
500 $a Adviser: M. M. Moloney.
502 $a Thesis (Ph.D.)--University of Calgary (Canada), 2003.
520 $a In the early 1990's, the Children's Vaccine Initiative was initiated to develop new technologies that would make vaccines cost-effective in both production and delivery. One interesting possibility offered by recent advances in immunology, and plant biotechnology is the oral delivery of subunit vaccines in the edible parts of a plant. Before edible vaccines become a practical approach in combating diseases, however, several concerns will have to be addressed including variability in antigen expression and the induction of oral tolerance.
520 $a Expressing antigens in seeds provides an alternative to that of expressing antigens in the edible part of plants including, the fact that recombinant proteins can accumulate to high levels, seeds are natural storage organs with limited protein degradation occurring, and seeds can be stored and transported at ambient temperature eliminating the need for refrigeration. The use of oilbodies for antigen accumulation and purification has the added advantages that an antigen coupled to oilbodies can be easily purified from other plant constituents and can be used as a delivery system.
520 $a The goal of my work was to evaluate the seed-based oleosin expression system as a novel means of antigen production and a novel delivery system. Three specific objectives were pursued, including the development of model oilbodies systems, the expression and accumulation of antigens on plant oilbodies, and the use of plant oilbodies as an antigen delivery system.
520 $a The major findings of this work included that proteins and peptides can be coupled to oilbodies by various approaches other than covalent coupling including using streptavidin-biotin and the intein-mediated protein ligation technologies. Second, prokaryotic genes have the potential to be aberrantly expressed in plants. However, they can also be properly expressed in plants and the antigens can accumulate to high levels on the surface of oilbodies. The antigens found on oilbodies can be functionally active as well as they may be partially buried within the oilbody. Third, oilbodies are not toxic and have no intrinsic adjuvant property. Mice immunized with an antigen coupled to plant oilbodies induce a greater antibody response against the antigen than mice immunized with antigen alone. The induction of an antibody response includes the induction of a memory response.
590 $a School code: 0026.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
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710 2 0 $a University of Calgary (Canada). $3 1017619
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790 1 0 $a Moloney, M. M., $e advisor
790 $a 0026
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ87013