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Factors that maintain cytosine methy...

Bartee, Lisa.

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Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana./
作者:	Bartee, Lisa.
面頁冊數:	216 p.
附註:	Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5521.
Contained By:	Dissertation Abstracts International65-11B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3155592
ISBN:	0496163124

Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana.
Bartee, Lisa.

Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana. - 216 p.

Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5521.

Thesis (Ph.D.)--The Johns Hopkins University, 2005.

In eukaryotes, including mammals, plants, and some fungi, cytosine methylation is a key regulator of transcriptional regulation and genome defense through the formation of compact heterochromatic regions. In both mammals and plants, the majority of cytosine methylation is found on repetitive sequences, but some single copy sequences are also methylated. This methylation is mainly in the symmetric dinucleotide context CG, but plants have additional cytosine methylation in CNG and asymmetric contexts. This thesis describes the effects of mutations in factors that control maintenance of cytosine methylation on the methylated endogenous family of PAI (p&barbelow;hosphoribosyla&barbelow;nthranilate i&barbelow;somerase) genes in Arabidopsis thaliana, which encode a tryptophan biosynthetic enzyme.

ISBN: 0496163124Subjects--Topical Terms:

1017730
Biology, Genetics.

Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana.
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245 1 0 $a Factors that maintain cytosine methylation on endogenous genes in Arabidopsis thaliana.
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500 $a Source: Dissertation Abstracts International, Volume: 65-11, Section: B, page: 5521.
500 $a Adviser: Judith Bender.
502 $a Thesis (Ph.D.)--The Johns Hopkins University, 2005.
520 $a In eukaryotes, including mammals, plants, and some fungi, cytosine methylation is a key regulator of transcriptional regulation and genome defense through the formation of compact heterochromatic regions. In both mammals and plants, the majority of cytosine methylation is found on repetitive sequences, but some single copy sequences are also methylated. This methylation is mainly in the symmetric dinucleotide context CG, but plants have additional cytosine methylation in CNG and asymmetric contexts. This thesis describes the effects of mutations in factors that control maintenance of cytosine methylation on the methylated endogenous family of PAI (p&barbelow;hosphoribosyla&barbelow;nthranilate i&barbelow;somerase) genes in Arabidopsis thaliana, which encode a tryptophan biosynthetic enzyme.
520 $a A novel reporter strain (pai1) was generated in which PAI silencing can be monitored by a blue fluorescent plant phenotype caused by accumulation of a tryptophan precursor compound. A forward genetic screen for factors that affect PAI silencing and methylation identified two major complementation groups: CMT3 and SUVH4, encoding a cytosine methyltransferase (C MTase) and a histone H3 lysine 9 methyltransferase (H3-K9 H MTase), respectively. Mutations in both genes cause reduced non-CG methylation on the PAI genes and other loci, with cmt3 showing a stronger phenotype than suvh4. The residual PAI methylation remaining in a cmt3 mutant, mostly in the symmetric dinucleotide CG context, can be lost at a low frequency and cannot be reestablished in the mutant background.
520 $a The pai1 strain was also used to explicitly test previously-identified mutations in the MET1 C MTase, which controls CG methylation, and the DDM1 chromatin remodeling factor, which controls both CG and non-CG methylation at some loci. The pai1 met1, pai1 ddm1, and pai1 met1 ddm1 strains all showed reduced, but not abolished, PAI methylation. However, PAI methylation was eliminated in a cmt3 met1 double mutant plant, implying that MET1 and CMT3 are the major C MTases required for maintenance of cytosine methylation on the PAI genes.
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