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Surimi wash water treatment by chito...

Wibowo, Singgih.

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Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment./
Author:	Wibowo, Singgih.
Description:	123 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5859.
Contained By:	Dissertation Abstracts International64-12B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116167
ISBN:	0496636030

Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.
Wibowo, Singgih.

Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment. - 123 p.

Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5859.

Thesis (Ph.D.)--Oregon State University, 2004.

Soluble surimi wash water (SWW) proteins could be recovered using chitosan (Chi) complexed with alginate (Chi-Alg) generating co-products for feed formulations. Chi with a degree of deacetylation (DD) of 84% complexed with Alg at a mixing ratio (MR) of 0.2 was used to study Chi-Alg concentration and treatment time protein recovery effects. Insoluble SWW solids were removed by centrifugation and the supernatant was then adjusted to pH 6. Flocculation at 20°C using Chi-Alg at 20, 40, 100 and 150 mg/L SWW was aided by 5 min agitation and holding for 30 min, 1h and 24h. Concentration had an effect between low (20 and 40 mg/L) and high (100 and 150 mg/L) levels. Time had an effect between 30 min and 1h but not between 1 and 24h. Turbidity reduction was affected only by concentration. 100 mg Chi-Alg/L SWW for 1h achieved 83% protein adsorption and 97% turbidity reduction while lower concentrations yielding higher adsorption required longer times. Fourier Transform Infrared (FTIR) analysis of untreated and Chi-Alg treated SWW solids confirmed protein adsorption. Amide band areas normalized against a common 3005--2880 cm-1 region confirmed the high protein recovery by 100 mg Chi-Alg/L SWW. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were tested to recover soluble SWW solids using 20, 40, and 100 mg Chi-Alg/L SWW (0.2 MR, 1h). High (94%, 93%) and low (75%) DD chitosan had lower protein adsorption (73--75%) when compared to the intermediate (84%) DD chitosan (74--83%). Intermediate DD and high MW Chi seemed to perform better; however, SY-1000 with 94% DD did not follow this trend (79--86% protein adsorption, 85--92% turbidity reduction).

ISBN: 0496636030Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.
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100 1 $a Wibowo, Singgih. $3 1925272
245 1 0 $a Surimi wash water treatment by chitosan-alginate complexes: Effect of molecular weight and degree of deacetylation of chitosan and nutritional evaluation of solids recovered by the treatment.
300 $a 123 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-12, Section: B, page: 5859.
500 $a Adviser: J. Antonio Torres.
502 $a Thesis (Ph.D.)--Oregon State University, 2004.
520 $a Soluble surimi wash water (SWW) proteins could be recovered using chitosan (Chi) complexed with alginate (Chi-Alg) generating co-products for feed formulations. Chi with a degree of deacetylation (DD) of 84% complexed with Alg at a mixing ratio (MR) of 0.2 was used to study Chi-Alg concentration and treatment time protein recovery effects. Insoluble SWW solids were removed by centrifugation and the supernatant was then adjusted to pH 6. Flocculation at 20°C using Chi-Alg at 20, 40, 100 and 150 mg/L SWW was aided by 5 min agitation and holding for 30 min, 1h and 24h. Concentration had an effect between low (20 and 40 mg/L) and high (100 and 150 mg/L) levels. Time had an effect between 30 min and 1h but not between 1 and 24h. Turbidity reduction was affected only by concentration. 100 mg Chi-Alg/L SWW for 1h achieved 83% protein adsorption and 97% turbidity reduction while lower concentrations yielding higher adsorption required longer times. Fourier Transform Infrared (FTIR) analysis of untreated and Chi-Alg treated SWW solids confirmed protein adsorption. Amide band areas normalized against a common 3005--2880 cm-1 region confirmed the high protein recovery by 100 mg Chi-Alg/L SWW. Six Chi samples differing in molecular weight (MW) and degree of deacetylation (DD) were tested to recover soluble SWW solids using 20, 40, and 100 mg Chi-Alg/L SWW (0.2 MR, 1h). High (94%, 93%) and low (75%) DD chitosan had lower protein adsorption (73--75%) when compared to the intermediate (84%) DD chitosan (74--83%). Intermediate DD and high MW Chi seemed to perform better; however, SY-1000 with 94% DD did not follow this trend (79--86% protein adsorption, 85--92% turbidity reduction).
520 $a Insoluble SWW (P1) and soluble solids (P2) recovered using 150 mg Chi-Alg/L SWW contained 61.4 and 73.1% protein, respectively. Rat diets formulated with 10% protein substitution by P1 and 10% and 15% by P2 had acceptability and protein efficiency ratios (PER) as high as the casein control with no deleterious effects. Rat diets with 100% P2 protein substitution showed higher PER and net protein ratio than the casein control with no deleterious effects. Protein recovered from SWW using Chi-Alg has the potential to be used in commercial feed formulations.
590 $a School code: 0172.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
690 $a 0359
710 2 0 $a Oregon State University. $3 625720
773 0 $t Dissertation Abstracts International $g 64-12B.
790 1 0 $a Torres, J. Antonio, $e advisor
790 $a 0172
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3116167