東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Overexpression analysis reveals nove...

Hennig, Krista Maria.

Linked to FindBook

Google Book

Amazon

博客來

Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway./
Author:	Hennig, Krista Maria.
Description:	224 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5525.
Contained By:	Dissertation Abstracts International67-10B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3239545
ISBN:	9780542942969

Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway.
Hennig, Krista Maria.

Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway. - 224 p.

Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5525.

Thesis (Ph.D.)--University of Minnesota, 2007.

To survive, develop, and reproduce in fluctuating environmental conditions, living organisms must tightly couple cell growth and metabolism to nutrient availability, cellular energy status, and growth factor signaling. The regulatory kinase Target of Rapamycin (TOR) has emerged as a convergence point for the transduction of multiple environmental and cellular signals into appropriate changes in growth and development.

ISBN: 9780542942969Subjects--Topical Terms:

1017719
Biology, Molecular.

Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway.
LDR:03547nmm 2200325 4500 001 1833769
005 20071112133558.5
008 130610s2007 eng d
020 $a 9780542942969
035 $a (UMI)AAI3239545
035 $a AAI3239545
040 $a UMI $c UMI
100 1 $a Hennig, Krista Maria. $3 1922452
245 1 0 $a Overexpression analysis reveals novel growth regulators in the target of rapamycin signaling pathway.
300 $a 224 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5525.
500 $a Adviser: Thomas P. Neufeld.
502 $a Thesis (Ph.D.)--University of Minnesota, 2007.
520 $a To survive, develop, and reproduce in fluctuating environmental conditions, living organisms must tightly couple cell growth and metabolism to nutrient availability, cellular energy status, and growth factor signaling. The regulatory kinase Target of Rapamycin (TOR) has emerged as a convergence point for the transduction of multiple environmental and cellular signals into appropriate changes in growth and development.
520 $a The following study was designed to further our understanding of nutrient-mediated growth regulation through analysis of TOR misexpression phenotypes and identification of novel interactors in the TOR signaling pathway. Utilizing the genetic tools of the model organism Drosophila melanogaster, we performed targeted overexpression analysis both to elucidate the downstream effects of TOR signaling, as well as to provide a sensitized genetic background in which to screen for novel TOR interactors.
520 $a I describe the effects of wild-type and kinase-inactive TOR overexpression on cell and organ size, cell cycle progression, apoptosis, and autophagy induction. The complex and progressive nature of TOR misexpression phenotypes, as well as the similarities between wild-type and kinase-inactive TOR overexpression, indicates the importance of regulated binding interactions in TOR signaling. The utility of tissue-specific TOR misexpression phenotypes as genetic backgrounds for dominant-modifier deficiency, insertional, and EMS mutagenesis screens is explored.
520 $a I describe the identification and analysis of two novel TOR interactors identified as dominant enhancers of eyeless-driven TOR overexpression phenotypes. The recovery of five alleles of Drosophila Tra1/TRRAP, a direct activator-binding subunit of histone acetlytransferase (HAT) complexes has revealed a novel role for Tral in growth regulation. Analysis of two alleles of Drosophila Hsc70-4 (clathrin-uncoating ATPase) has revealed that TOR acts to mediate nutrient import, in part, by coordinately regulating bulk endocytic uptake and the targeted endocytic turnover of nutrient transporters such as Slimfast. We find that disruption of endocytosis affects cell size, cell cycle phasing, P13kinase signaling, autophagy induction, as well as tsc1 and TOR mutant phenotypes, indicating a critical role for cellular endocytosis in mediating proper growth regulation. These studies have broadened our understanding of the mechanisms through which eukaryotic organisms achieve a directed growth response appropriate to available nutrients, energy, and growth factor signaling.
590 $a School code: 0130.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Cell. $3 1017686
690 $a 0307
690 $a 0369
690 $a 0379
710 2 0 $a University of Minnesota. $3 676231
773 0 $t Dissertation Abstracts International $g 67-10B.
790 1 0 $a Neufeld, Thomas P., $e advisor
790 $a 0130
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3239545