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Cataracts in the fat sand rat: An oc...

DiCarlo, Cheryl Dawn.

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Cataracts in the fat sand rat: An ocular complication of diabetes.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Cataracts in the fat sand rat: An ocular complication of diabetes./
作者:	DiCarlo, Cheryl Dawn.
面頁冊數:	184 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5621.
Contained By:	Dissertation Abstracts International63-12B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3074863
ISBN:	9780493949383

Cataracts in the fat sand rat: An ocular complication of diabetes.
DiCarlo, Cheryl Dawn.

Cataracts in the fat sand rat: An ocular complication of diabetes. - 184 p.

Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5621.

Thesis (Ph.D.)--Uniformed Services University of the Health Sciences, 2001.

Purpose. The fat sand rat, Psammomys obesus , a spontaneous model for Type 2 diabetes, develops cataracts within days of the onset of clinical diabetes. The purpose of this research was to determine the crystallin composition of the normal sand rat lens and determine the changes that occur during diabetic cataract formation.

ISBN: 9780493949383Subjects--Topical Terms:

1017686
Biology, Cell.

Cataracts in the fat sand rat: An ocular complication of diabetes.
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245 1 0 $a Cataracts in the fat sand rat: An ocular complication of diabetes.
300 $a 184 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5621.
500 $a Adviser: Diane E. Borst.
502 $a Thesis (Ph.D.)--Uniformed Services University of the Health Sciences, 2001.
520 $a Purpose. The fat sand rat, Psammomys obesus , a spontaneous model for Type 2 diabetes, develops cataracts within days of the onset of clinical diabetes. The purpose of this research was to determine the crystallin composition of the normal sand rat lens and determine the changes that occur during diabetic cataract formation.
520 $a Materials and methods. Slit-lamp biomicroscopy was used for ocular examinations. Blood glucose, glycated hemoglobin (HbA1c), aqueous glucose, vitreous glucose, vitreous glycated protein and lens extract glycated protein and protein values were determined using standard techniques and colorimetric test kits. Location of glucose transporter (GLUT) transcripts was determined using reverse transcriptase polymerase chain reaction (rtPCR). Lens extracts were prepared then separated by gel filtration chromatography to study the crystallin classes by SDS-PAGE. Western blotting and mass spectrometry were used to verify the crystallin composition.
520 $a Results. Blood glucose, aqueous glucose, vitreous glucose and glycated hemoglobin (HbA1c) levels were all significantly elevated in the diabetic sand rats. Water-soluble protein levels were higher in the control sand rats. No significant differences were seen among glycated protein levels of the lens extracts. GLUT1, GLUT3 and GLUT4 transcripts were present in the anterior epithelial cells of the lens but only the GLUT4 transcript was present in the lens fiber cells. SDS-PAGE of the diabetic animals revealed an increased banding pattern at 35--42 kDa and a band shift from 22.5 to 23.5 kDa. Western blotting and mass spectrometry confirmed alpha-A crystallin aggregates and betaB2/betaB3 crystallin band shifts, respectively.
520 $a Discussion. This is the first report of characterization of the normal and cataractous lens in the fat sand rat. The elevated glucose and HbA1c values in the blood of diabetic sand rats result in high levels of glucose within the eye. In the diabetics, there are alphaA crystallin aggregates and betaB2/betaB3 crystallin ratio shifts with cataracts. The diabetic cataracts seen on the slit-lamp exam are similar to those seen in humans. The data reported here provides the groundwork for medical therapies aimed at reversal or prevention of diabetic cataracts instead of surgical alleviation of this common cause of blindness. The sand rat is an excellent choice for such diabetes research.
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650 4 $a Biology, Animal Physiology. $3 1017835
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773 0 $t Dissertation Abstracts International $g 63-12B.
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791 $a Ph.D.
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