東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Maps, myelin, and you: Genetic anal...

Woods, Ian Gregory.

Linked to FindBook

Google Book

Amazon

博客來

Maps, myelin, and you: Genetic analyses of vertebrate development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Maps, myelin, and you: Genetic analyses of vertebrate development./
Author:	Woods, Ian Gregory.
Description:	216 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5796.
Contained By:	Dissertation Abstracts International66-11B.
Subject:	Biology, Neuroscience. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3197529
ISBN:	9780542432279

Maps, myelin, and you: Genetic analyses of vertebrate development.
Woods, Ian Gregory.

Maps, myelin, and you: Genetic analyses of vertebrate development. - 216 p.

Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5796.

Thesis (Ph.D.)--Stanford University, 2006.

Genetic screens in zebrafish have identified thousands of mutations in hundreds of essential genes. Here we present an extensive genetic map for the zebrafish, which has facilitated the molecular identification of mutations. We employ our map in comparative genomic analyses to study aspects of vertebrate genome evolution, including identification of conserved syntenic regions, analyses of gene and genome duplication, delineation of the ancestral vertebrate karyotype, and illumination of gene content within ancestral chromosomes.

ISBN: 9780542432279Subjects--Topical Terms:

1017680
Biology, Neuroscience.

Maps, myelin, and you: Genetic analyses of vertebrate development.
LDR:03347nmm 2200313 4500 001 1831983
005 20070628102621.5
008 130610s2006 eng d
020 $a 9780542432279
035 $a (UnM)AAI3197529
035 $a AAI3197529
040 $a UnM $c UnM
100 1 $a Woods, Ian Gregory. $3 1920742
245 1 0 $a Maps, myelin, and you: Genetic analyses of vertebrate development.
300 $a 216 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5796.
500 $a Adviser: William S. Talbot.
502 $a Thesis (Ph.D.)--Stanford University, 2006.
520 $a Genetic screens in zebrafish have identified thousands of mutations in hundreds of essential genes. Here we present an extensive genetic map for the zebrafish, which has facilitated the molecular identification of mutations. We employ our map in comparative genomic analyses to study aspects of vertebrate genome evolution, including identification of conserved syntenic regions, analyses of gene and genome duplication, delineation of the ancestral vertebrate karyotype, and illumination of gene content within ancestral chromosomes.
520 $a Screens in zebrafish have facilitated analysis of conserved signaling pathways essential for development. Hedgehog signaling controls specification of many cell types and can lead to cancer when misregulated. In zebrafish, disruptions in Hedgehog signaling cause a U-shaped somite phenotype; consequently, these mutants have been designated as "you-class". We show that the extracellular EGF-CUB gene scube2 is disrupted in the eponymous you-class mutant, you, and is required downstream of Hedgehog transcription and upstream of Hedgehog reception. Analysis of genetic chimeras indicates that you is required neither in cells sending nor in cells receiving Hedgehog signals. Our data instead support a role for you in stabilization or transport of Hedgehog.
520 $a Screens for disruptions in myelin gene expression have identified mutations in several genes essential for myelination. We show that two of these mutations disrupt nsf, an ATPase with critical roles in membrane fusion and synaptic transmission. Zebrafish nsf mutants are paralyzed and visually impaired, reflecting the requirement of nsf in synaptic transmission and neural activity. Strikingly, sodium channels fail to cluster along myelinated axons at the nodes of Ranvier in mutants. Pharmacological inhibition of neural activity and chemical synaptic transmission indicate that these roles of nsf are not required for sodium channel clustering. Instead, we have uncovered a novel function of nsf, independent of its synaptic role, in organizing nodes of Ranvier.
520 $a Reverse genetic techniques are less advanced than forward genetic approaches in zebrafish. We employ a sequencing based approach to identify heterozygous carriers of mutations in specific genes. Using these methods, we generated zebrafish with disrupted function of the deltac gene, and have thereby uncovered an essential role for deltac in somitogenesis.
590 $a School code: 0212.
650 4 $a Biology, Neuroscience. $3 1017680
650 4 $a Biology, Genetics. $3 1017730
690 $a 0317
690 $a 0369
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 66-11B.
790 1 0 $a Talbot, William S., $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3197529