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Implementation and evaluation of a m...

Codrea, Felicia.

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Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics./
Author:	Codrea, Felicia.
Description:	109 p.
Notes:	Source: Masters Abstracts International, Volume: 44-06, page: 2800.
Contained By:	Masters Abstracts International44-06.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1434339
ISBN:	9780542678820

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
Codrea, Felicia.

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics. - 109 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2800.

Thesis (M.S.)--Michigan State University, 2006.

Although "gene therapy" is a concept that was introduced more than there decades ago, the successes of this method are below the expectations. The bottleneck of gene therapy is gene delivery. The current non-viral methods used for transfection possess low transfection efficiency and are not suitable for therapeutic use, showing high toxicity.

ISBN: 9780542678820Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
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008 130610s2006 eng d
020 $a 9780542678820
035 $a (UnM)AAI1434339
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100 1 $a Codrea, Felicia. $3 1920712
245 1 0 $a Implementation and evaluation of a molecular strategy for delivery of nucleic acid based therapeutics.
300 $a 109 p.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2800.
500 $a Adviser: Rawle I. Hollingsworth.
502 $a Thesis (M.S.)--Michigan State University, 2006.
520 $a Although "gene therapy" is a concept that was introduced more than there decades ago, the successes of this method are below the expectations. The bottleneck of gene therapy is gene delivery. The current non-viral methods used for transfection possess low transfection efficiency and are not suitable for therapeutic use, showing high toxicity.
520 $a A new macromolecular scaffold that combines the properties of cationic polymers and cationic lipids was synthesized in only two steps. It contains a lipophilic part, like lipids, and a charged amine moiety, like cationic polymers. A polymeric backbone was synthesized through a Michael addition reaction, starting from 2(5H)-furanone and cysteine. After purification through membrane fractionation, the backbone was functionalized with N, N dimethylethylenediamine and octylamine. The ability of this new carrier to mediate transfection of plasmid encoding for green fluorescent protein in vitro was tested on COS 1 cells. The transfection conditions were optimized and an efficiency of transfection of 6.59% was achieved. The evaluation of the cytotoxicity of this new carrier proved that the carrier is non-toxic. The cells proliferated up to confluence even when the concentration was ten times higher than what was identified as optimum for transfection.
590 $a School code: 0128.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Pharmaceutical. $3 550957
690 $a 0487
690 $a 0491
710 2 0 $a Michigan State University. $3 676168
773 0 $t Masters Abstracts International $g 44-06.
790 1 0 $a Hollingsworth, Rawle I., $e advisor
790 $a 0128
791 $a M.S.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1434339