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Characterization and functional asse...

Kelley-Clarke, Brenna Kathleen.

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Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain./
Author:	Kelley-Clarke, Brenna Kathleen.
Description:	186 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2371.
Contained By:	Dissertation Abstracts International67-05B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3217783
ISBN:	9780542693007

Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain.
Kelley-Clarke, Brenna Kathleen.

Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain. - 186 p.

Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2371.

Thesis (Ph.D.)--Harvard University, 2006.

Kaposi's Sarcoma associated herpesvirus (KSHV) establishes latent infection in dividing cells. To persist, viral episomes must replicate and properly segregate to progeny nuclei. Latency-associated nuclear antigen (LANA) mediates viral persistence by tethering episomes to chromosomes during mitosis. LANA contains two independent chromosome association regions located in the protein's N- and C-terminal regions. N-terminal LANA chromosome binding is necessary for both episome replication and persistence. However, little is known about C-terminal LANA chromosome binding. In this dissertation, we have used mutational analysis to characterize the C-terminal LANA chromosome binding region and investigate its role in episome persistence. We demonstrate that C-terminal LANA concentrates to paired dots at pericentromeric and peri-telomeric regions of a subset of mitotic chromosomes through residues 996-1139. A deletion abolishing specific DNA binding did not affect chromosome targeting, indicating that LANA's localization does not occur by direct binding to chromosomal DNA. Instead, C-terminal LANA chromosome binding likely occurs by interaction with a cell factor(s) that concentrates at pericentromeric and peri-telomeric chromosome regions.

ISBN: 9780542693007Subjects--Topical Terms:

1017719
Biology, Molecular.

Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain.
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100 1 $a Kelley-Clarke, Brenna Kathleen. $3 1917909
245 1 0 $a Characterization and functional assessment of the Kaposi's sarcoma herpesvirus latency-associated nuclear antigen C-terminal chromosome binding domain.
300 $a 186 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-05, Section: B, page: 2371.
500 $a Adviser: Kenneth Kaye.
502 $a Thesis (Ph.D.)--Harvard University, 2006.
520 $a Kaposi's Sarcoma associated herpesvirus (KSHV) establishes latent infection in dividing cells. To persist, viral episomes must replicate and properly segregate to progeny nuclei. Latency-associated nuclear antigen (LANA) mediates viral persistence by tethering episomes to chromosomes during mitosis. LANA contains two independent chromosome association regions located in the protein's N- and C-terminal regions. N-terminal LANA chromosome binding is necessary for both episome replication and persistence. However, little is known about C-terminal LANA chromosome binding. In this dissertation, we have used mutational analysis to characterize the C-terminal LANA chromosome binding region and investigate its role in episome persistence. We demonstrate that C-terminal LANA concentrates to paired dots at pericentromeric and peri-telomeric regions of a subset of mitotic chromosomes through residues 996-1139. A deletion abolishing specific DNA binding did not affect chromosome targeting, indicating that LANA's localization does not occur by direct binding to chromosomal DNA. Instead, C-terminal LANA chromosome binding likely occurs by interaction with a cell factor(s) that concentrates at pericentromeric and peri-telomeric chromosome regions.
590 $a School code: 0084.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
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773 0 $t Dissertation Abstracts International $g 67-05B.
790 1 0 $a Kaye, Kenneth, $e advisor
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