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Tracking neuronal content using capi...

Wise, Dana Diane.

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Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence./
作者:	Wise, Dana Diane.
面頁冊數:	127 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4201.
Contained By:	Dissertation Abstracts International66-08B.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3184808
ISBN:	9780542259982

Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence.
Wise, Dana Diane.

Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence. - 127 p.

Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4201.

Thesis (Ph.D.)--The University of Texas at Austin, 2005.

Capillary electrophoresis with multiphoton-excited fluorescence detection (CE-MPE) allows low-background analysis of many spectrally distinct biological fluorophores using a single long-wavelength laser. This work demonstrates the methodical transformation of CE-MPE from a proof-of-concept instrument to a reliable and powerful workhorse for complex cellular samples. Preparation of cell extracts and their long-term storage prior to CE-MPE analysis have also been exhaustively characterized (Chapter 4). The process is suitable for extractions at 2 to 3 hour intervals over a day or more, or as frequently as every hour for shorter durations. With these methods, answers were obtained for hypothesis-driven research---answers not readily available from other techniques.

ISBN: 9780542259982Subjects--Topical Terms:

586156
Chemistry, Analytical.

Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence.
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245 1 0 $a Tracking neuronal content using capillary electrophoresis with multiphoton excitation of fluorescence.
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500 $a Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4201.
500 $a Supervisor: Jason B. Shear.
502 $a Thesis (Ph.D.)--The University of Texas at Austin, 2005.
520 $a Capillary electrophoresis with multiphoton-excited fluorescence detection (CE-MPE) allows low-background analysis of many spectrally distinct biological fluorophores using a single long-wavelength laser. This work demonstrates the methodical transformation of CE-MPE from a proof-of-concept instrument to a reliable and powerful workhorse for complex cellular samples. Preparation of cell extracts and their long-term storage prior to CE-MPE analysis have also been exhaustively characterized (Chapter 4). The process is suitable for extractions at 2 to 3 hour intervals over a day or more, or as frequently as every hour for shorter durations. With these methods, answers were obtained for hypothesis-driven research---answers not readily available from other techniques.
520 $a For example, evidence suggested intracellular levels of vitamin B 3 (nicotinamide) derivatives might exhibit a circadian rhythm in suprachiasmatic nuclei neurons. Therefore, Chapter 4 presents the tracking of these cofactors over 24--48 h periods in extracts prepared from an immortalized biological clock cell line. Chapter 5 extends this single-fluorophore work to investigate hypothesized intracellular changes in both indole and nicotinamide derivatives during depolarization-induced upregulation of serotonergic phenotype, using cells immortalized from the raphe nuclei of the brain. Chapter 5 also demonstrates detection of riboflavin (vitamin B2) derivatives in cell extracts, and proposes several relevant continuation experiments. Finally, Chapter 6 broadens the capabilities of CE-MPE to neutral analytes, such as melatonin, for the circadian investigation of multiple analytes in cells immortalized from the pineal gland, another clock-like area of the brain.
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