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The photocycle of photoactive yellow...

Bao, Qinan.

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The photocycle of photoactive yellow protein.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The photocycle of photoactive yellow protein./
Author:	Bao, Qinan.
Description:	109 p.
Notes:	Source: Masters Abstracts International, Volume: 43-03, page: 0795.
Contained By:	Masters Abstracts International43-03.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1424147
ISBN:	9780496140251

The photocycle of photoactive yellow protein.
Bao, Qinan.

The photocycle of photoactive yellow protein. - 109 p.

Source: Masters Abstracts International, Volume: 43-03, page: 0795.

Thesis (M.S.)--Oklahoma State University, 2005.

Scope and method of study. We studied the photocycle of Photoactive Yellow Protein (PYP).

ISBN: 9780496140251Subjects--Topical Terms:

1019105
Biophysics, General.

The photocycle of photoactive yellow protein.
LDR:02389nmm 2200301 4500 001 1823970
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008 130610s2005 eng d
020 $a 9780496140251
035 $a (UnM)AAI1424147
035 $a AAI1424147
040 $a UnM $c UnM
100 1 $a Bao, Qinan. $3 1913064
245 1 4 $a The photocycle of photoactive yellow protein.
300 $a 109 p.
500 $a Source: Masters Abstracts International, Volume: 43-03, page: 0795.
500 $a Adviser: Aihua Xie.
502 $a Thesis (M.S.)--Oklahoma State University, 2005.
520 $a Scope and method of study. We studied the photocycle of Photoactive Yellow Protein (PYP).
520 $a Findings and conclusions. We performed exponential fitting with different numbers of exponentials on the flash photolysis data at 11 temperatures. By comparing the standard deviations with the numbers of exponentials used, we concluded that 4 exponentials are most appropriate to fit the flash photolysis data. Therefore, the PYP photocycle is best described with 4 intermediates according to the first-order kinetics theorem. In additional to p R, pB' and pB, there is an intermediate state PYPN formed during the recovery of pG from pB. Additional studies are needed to characterize this intermediate. The global analysis of the flash photolysis data prompted a further experimental investigation of the temperature dependence of the pG absorption in the UV/vis region. We demonstrated that the pG absorption is strongly temperature dependent. This impedes the multi-temperature global modeling of the flash photolysis data. We also found that as temperature increases, pB dark is formed. By analyzing the FTIR stepscan data, the bi-exponential properties of the 1726 cm-1 and 1514--1498 cm -1 bands proved the existence of the back reaction from pB' to pR. With population model fitting, we obtained the actual rate constants for the PYP intermediates transitions at 300 K for our new photocycle model. Our new photocycle model contains an additional intermediate PYPN and one back reaction from pB' to pR. (Abstract shortened by UMI.)
590 $a School code: 0664.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Physics, Molecular. $3 1018648
690 $a 0786
690 $a 0487
690 $a 0609
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Masters Abstracts International $g 43-03.
790 1 0 $a Xie, Aihua, $e advisor
790 $a 0664
791 $a M.S.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1424147