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Heterogeneous patterns of phagosome ...

Henry, Rebecca M.

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Heterogeneous patterns of phagosome maturation in macrophages.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Heterogeneous patterns of phagosome maturation in macrophages./
Author:	Henry, Rebecca M.
Description:	160 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0796.
Contained By:	Dissertation Abstracts International66-02B.
Subject:	Health Sciences, Immunology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3163818
ISBN:	9780496981601

Heterogeneous patterns of phagosome maturation in macrophages.
Henry, Rebecca M.

Heterogeneous patterns of phagosome maturation in macrophages. - 160 p.

Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0796.

Thesis (Ph.D.)--University of Michigan, 2005.

Phagocytosis is a fundamental cellular process that serves multiple functions in tissue homeostasis and immunity. Complex signaling pathways regulate the engulfment of particles into phagosomes or vacuoles and the sequential interactions of phagosomes with the endocytic pathway, a process called phagosome maturation. The final step of maturation is the degradation of internalized particles within lysosomes. Phagosome maturation is presumed to be identical for a single kind of internalized particle. However, this presumption has not been tested. We therefore tested the hypothesis that all phagosomes containing a single kind of particle mature identically.

ISBN: 9780496981601Subjects--Topical Terms:

1017716
Health Sciences, Immunology.

Heterogeneous patterns of phagosome maturation in macrophages.
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035 $a (UnM)AAI3163818
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100 1 $a Henry, Rebecca M. $3 1912967
245 1 0 $a Heterogeneous patterns of phagosome maturation in macrophages.
300 $a 160 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-02, Section: B, page: 0796.
500 $a Chair: Joel A. Swanson.
502 $a Thesis (Ph.D.)--University of Michigan, 2005.
520 $a Phagocytosis is a fundamental cellular process that serves multiple functions in tissue homeostasis and immunity. Complex signaling pathways regulate the engulfment of particles into phagosomes or vacuoles and the sequential interactions of phagosomes with the endocytic pathway, a process called phagosome maturation. The final step of maturation is the degradation of internalized particles within lysosomes. Phagosome maturation is presumed to be identical for a single kind of internalized particle. However, this presumption has not been tested. We therefore tested the hypothesis that all phagosomes containing a single kind of particle mature identically.
520 $a The maturation pathways followed by phagosomes containing IgG-opsonized erythrocytes (E-IgG) in living macrophages were determined using ratiometric fluorescence microscopy. Analysis of individual phagosomes revealed that they exhibited heterogeneous patterns of phagosome maturation. While the dynamics of most endocytic markers on E-IgG phagosomal membranes were consistent, the acquisition and loss of the lipid phosphatidylinositol 3-phosphate were heterogeneous. The heterogeneity reflected the amplification of small differences in intracellular signals generated by phagosomes and the integration of signals over the entire phagosome.
520 $a Many pathogens manipulate the signaling pathways involved in phagocytosis to avoid degradation and to establish intracellular infections within phagocytes such as macrophages. The intracellular pathogen Listeria monocytogenes evades the microbicidal mechanisms of macrophages by escaping from vacuoles to the cytosol. While many bacteria escape their vacuoles, others do not. The variability in L. monocytogenes escape was investigated using a YFP-labeled indicator of L. monocytogenes entry to the cytosol and CFP-labeled endocytic membrane markers. L. monocytogenes that delayed fusion with lysosomes escaped late endosome-stage vacuoles. Other L. monocytogenes-containing vacuoles merged with lysosomes, where escape was inhibited. Listeriolysin O (LLO), a cytolysin produced by L. monocytogenes, was responsible for delaying vacuole fusion with lysosomes. The manipulation of vacuole maturation by L. monocytogenes enhanced escape to the cytoplasm. Variability in L. monocytogenes escape may have been a result of heterogeneity in the timing of LLO activity.
520 $a These studies revealed that identical phagosomes do not always follow identical maturation pathways in macrophages. Phagosomes must be analyzed individually to observe heterogeneous aspects of their maturation.
590 $a School code: 0127.
650 4 $a Health Sciences, Immunology. $3 1017716
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Cell. $3 1017686
690 $a 0982
690 $a 0410
690 $a 0379
710 2 0 $a University of Michigan. $3 777416
773 0 $t Dissertation Abstracts International $g 66-02B.
790 1 0 $a Swanson, Joel A., $e advisor
790 $a 0127
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3163818