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Transformation of American elm with ...

Newhouse, Andrew E.

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Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease./
Author:	Newhouse, Andrew E.
Description:	97 p.
Notes:	Source: Masters Abstracts International, Volume: 44-04, page: 1722.
Contained By:	Masters Abstracts International44-04.
Subject:	Agriculture, Forestry and Wildlife. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430685
ISBN:	9780542479397

Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
Newhouse, Andrew E.

Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease. - 97 p.

Source: Masters Abstracts International, Volume: 44-04, page: 1722.

Thesis (M.S.)--State University of New York College of Environmental Science and Forestry, 2005.

American elms were a nearly ubiquitous part of both urban and rural life in the United States until Dutch-elm disease, caused by the introduced fungus Ophiostoma novo-ulmi, killed almost all mature trees in the mid-twentieth century. In this project, Agrobacterium was used to transform American elm with a vascular-expressed gene encoding the synthetic antimicrobial peptide ESF39A. At least three unique, single-copy transgenic lines were produced and regenerated into whole plants, which showed significantly less wilting and sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Copy number was determined by Southern hybridization, expression was confirmed by RT-PCR, and peptide activity against O. novo-ulmi was tested with an in vitro 'minimum inhibitory concentration' assay. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. These results indicate that transgenes encoding antimicrobial peptides hold promise for enhancing pathogen resistance in American elm.

ISBN: 9780542479397Subjects--Topical Terms:

783690
Agriculture, Forestry and Wildlife.

Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
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245 1 0 $a Transformation of American elm with a gene encoding a synthetic antimicrobial peptide for resistance to Dutch-elm disease.
300 $a 97 p.
500 $a Source: Masters Abstracts International, Volume: 44-04, page: 1722.
500 $a Adviser: William Powell.
502 $a Thesis (M.S.)--State University of New York College of Environmental Science and Forestry, 2005.
520 $a American elms were a nearly ubiquitous part of both urban and rural life in the United States until Dutch-elm disease, caused by the introduced fungus Ophiostoma novo-ulmi, killed almost all mature trees in the mid-twentieth century. In this project, Agrobacterium was used to transform American elm with a vascular-expressed gene encoding the synthetic antimicrobial peptide ESF39A. At least three unique, single-copy transgenic lines were produced and regenerated into whole plants, which showed significantly less wilting and sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Copy number was determined by Southern hybridization, expression was confirmed by RT-PCR, and peptide activity against O. novo-ulmi was tested with an in vitro 'minimum inhibitory concentration' assay. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. These results indicate that transgenes encoding antimicrobial peptides hold promise for enhancing pathogen resistance in American elm.
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