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Gene delivery modulated by redox pot...

Soundara Manickam, Devika.

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Gene delivery modulated by redox potential gradients.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Gene delivery modulated by redox potential gradients./
Author:	Soundara Manickam, Devika.
Description:	109 p.
Notes:	Source: Masters Abstracts International, Volume: 44-03, page: 1376.
Contained By:	Masters Abstracts International44-03.
Subject:	Chemistry, Pharmaceutical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430529
ISBN:	9780542434341

Gene delivery modulated by redox potential gradients.
Soundara Manickam, Devika.

Gene delivery modulated by redox potential gradients. - 109 p.

Source: Masters Abstracts International, Volume: 44-03, page: 1376.

Thesis (M.S.)--Wayne State University, 2005.

The existence of a high redox potential gradient across the cellular membranes offers an advantageous stimulus for the programmed delivery of genes. In this project, we have synthesized vectors sensitive to redox potential gradients, which have proven their ability to deliver genes with high transfection efficiency and minimal cytotoxicity. Bioactive peptide sequences have demonstrated their natural ability to translocate across cell membranes and to deliver DNA to the nucleus of the target cells. A high molecular weight homopolypeptide was synthesized based on the TAT peptide; a protein transduction domain derived from the transactivating transcriptional activator protein from HIV-1 and for the first time, a high molecular weight copolypeptide was synthesized by randomly linking two different peptide sequences: a nuclear localization sequence peptide derived from the SV40 virus and a histidine rich buffering peptide. Physical properties and biological activity of the polycation/DNA complexes were studied. The results demonstrate that carriers based on these bioactive peptide sequences possess high transfection activity in the tested cell lines and exhibit minimal to negligible cytotoxicity in the human endothelial cells. These observations encourage examining the suitability of these carriers for in vivo gene delivery. (Abstract shortened by UMI.)

ISBN: 9780542434341Subjects--Topical Terms:

550957
Chemistry, Pharmaceutical.

Gene delivery modulated by redox potential gradients.
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520 $a The existence of a high redox potential gradient across the cellular membranes offers an advantageous stimulus for the programmed delivery of genes. In this project, we have synthesized vectors sensitive to redox potential gradients, which have proven their ability to deliver genes with high transfection efficiency and minimal cytotoxicity. Bioactive peptide sequences have demonstrated their natural ability to translocate across cell membranes and to deliver DNA to the nucleus of the target cells. A high molecular weight homopolypeptide was synthesized based on the TAT peptide; a protein transduction domain derived from the transactivating transcriptional activator protein from HIV-1 and for the first time, a high molecular weight copolypeptide was synthesized by randomly linking two different peptide sequences: a nuclear localization sequence peptide derived from the SV40 virus and a histidine rich buffering peptide. Physical properties and biological activity of the polycation/DNA complexes were studied. The results demonstrate that carriers based on these bioactive peptide sequences possess high transfection activity in the tested cell lines and exhibit minimal to negligible cytotoxicity in the human endothelial cells. These observations encourage examining the suitability of these carriers for in vivo gene delivery. (Abstract shortened by UMI.)
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