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Use of polymerase chain reaction bas...

Li, Mei.

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Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets./
Author:	Li, Mei.
Description:	115 p.
Notes:	Source: Masters Abstracts International, Volume: 43-01, page: 0125.
Contained By:	Masters Abstracts International43-01.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ92963
ISBN:	9780612929630

Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets.
Li, Mei.

Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets. - 115 p.

Source: Masters Abstracts International, Volume: 43-01, page: 0125.

Thesis (M.Sc.)--University of Guelph (Canada), 2004.

Techniques were developed to collect and prepare microbial samples from intestinal digesta and mucosal surfaces from early-weaned piglets. The washing procedure was developed for preparation of bacteria attached to the mucosal surface. A new commercial kit (QIAampRTM, QIAGEN) was proved to be effective for DNA extraction from intestinal microflora in pigs. A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method demonstrated a high level of reproducibility and sensitivity for characterization of pig intestinal microflora. Effects of including antibiotics, two acid mixtures and one herbal extract product in the diet on growth performance and intestinal microflora diversity were investigated using piglets weaned at 16 days of age. Supplementation with acid mixture-2 (containing 1% lactic acid) increased average daily gain (ADG) as compared to the non-medicated control (P < 0.05) and brought it to a level similar to the medicated control (P > 0.05) at week 2 post-weaning. Supplementation with these feed additives inhibited a range of intestinal bacteria. In addition, the ileal microflora exhibited a lower diversity than those in the cecum and colon. Diversity of the intestinal microflora increased from weaning to day 14 post-weaning.

ISBN: 9780612929630Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets.
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245 1 0 $a Use of polymerase chain reaction based methods to characterize microflora in newly weaned piglets.
300 $a 115 p.
500 $a Source: Masters Abstracts International, Volume: 43-01, page: 0125.
500 $a Advisers: C. F. M. de Lange; J. Gong.
502 $a Thesis (M.Sc.)--University of Guelph (Canada), 2004.
520 $a Techniques were developed to collect and prepare microbial samples from intestinal digesta and mucosal surfaces from early-weaned piglets. The washing procedure was developed for preparation of bacteria attached to the mucosal surface. A new commercial kit (QIAampRTM, QIAGEN) was proved to be effective for DNA extraction from intestinal microflora in pigs. A polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method demonstrated a high level of reproducibility and sensitivity for characterization of pig intestinal microflora. Effects of including antibiotics, two acid mixtures and one herbal extract product in the diet on growth performance and intestinal microflora diversity were investigated using piglets weaned at 16 days of age. Supplementation with acid mixture-2 (containing 1% lactic acid) increased average daily gain (ADG) as compared to the non-medicated control (P < 0.05) and brought it to a level similar to the medicated control (P > 0.05) at week 2 post-weaning. Supplementation with these feed additives inhibited a range of intestinal bacteria. In addition, the ileal microflora exhibited a lower diversity than those in the cecum and colon. Diversity of the intestinal microflora increased from weaning to day 14 post-weaning.
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650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
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710 2 0 $a University of Guelph (Canada). $3 1018650
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790 1 0 $a de Lange, C. F. M., $e advisor
790 1 0 $a Gong, J., $e advisor
790 $a 0081
791 $a M.Sc.
792 $a 2004
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