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High performance liquid chromatograp...

Musukula, Sudheer Reddy.

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High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices.

Record Type:	Electronic resources : Monograph/item
Title/Author:	High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices./
Author:	Musukula, Sudheer Reddy.
Description:	107 p.
Notes:	Source: Masters Abstracts International, Volume: 44-03, page: 1264.
Contained By:	Masters Abstracts International44-03.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1430550
ISBN:	9780542436994

High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices.
Musukula, Sudheer Reddy.

High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices. - 107 p.

Source: Masters Abstracts International, Volume: 44-03, page: 1264.

Thesis (M.S.)--South Dakota State University, 2005.

5-Methyltetrahyrofolate (5-MeTHF) was analyzed in food samples using reverse phase chromatography, a gradient mobile phase of 33 mM phosphate buffer (pH 2.3) and acetonitrile, a flow rate 0.8ml/min, and fluorometric detection. For natural folate analysis, procedures were optimized for thermal extraction, enzymatic deconjugation, and clean up protocol using a strong anion exchange columns (SAX) and affinity chromatography using folate bind protein (FBP) columns. SAX column clean up did not yield clearly resolved peaks for broccoli. However, affinity column clean up yielded well resolved peaks. While the peak areas were small, they were still quantifiable. The 5-MeTHF content in spinach assayed using affinity chromatography for sample clean-up was lower than the concentration determined using Solid phase extraction (SPE) columns (15.66+/-0.83 vs. 98.92+/-1.24 mug/100 g). Results from SAX column clean up were closer to LCMS values than that of FBP clean up values. (Abstract shortened by UMI.)

ISBN: 9780542436994Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices.
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245 1 0 $a High performance liquid chromatographic method to determine natural folate (5-methyltetrahydrofolate) and added folic acid content in food matrices.
300 $a 107 p.
500 $a Source: Masters Abstracts International, Volume: 44-03, page: 1264.
500 $a Adviser: Padmanaban G. Krishnan.
502 $a Thesis (M.S.)--South Dakota State University, 2005.
520 $a 5-Methyltetrahyrofolate (5-MeTHF) was analyzed in food samples using reverse phase chromatography, a gradient mobile phase of 33 mM phosphate buffer (pH 2.3) and acetonitrile, a flow rate 0.8ml/min, and fluorometric detection. For natural folate analysis, procedures were optimized for thermal extraction, enzymatic deconjugation, and clean up protocol using a strong anion exchange columns (SAX) and affinity chromatography using folate bind protein (FBP) columns. SAX column clean up did not yield clearly resolved peaks for broccoli. However, affinity column clean up yielded well resolved peaks. While the peak areas were small, they were still quantifiable. The 5-MeTHF content in spinach assayed using affinity chromatography for sample clean-up was lower than the concentration determined using Solid phase extraction (SPE) columns (15.66+/-0.83 vs. 98.92+/-1.24 mug/100 g). Results from SAX column clean up were closer to LCMS values than that of FBP clean up values. (Abstract shortened by UMI.)
590 $a School code: 0205.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
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710 2 0 $a South Dakota State University. $3 1019395
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