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Identification of microorganisms in ...

Luo, Hongliang.

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Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development./
Author:	Luo, Hongliang.
Description:	212 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5743.
Contained By:	Dissertation Abstracts International66-11B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3197834
ISBN:	9780542421600

Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development.
Luo, Hongliang.

Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development. - 212 p.

Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5743.

Thesis (Ph.D.)--The Ohio State University, 2005.

Most foods can be considered as ecosystems containing various microorganisms including pathogenic, spoilage, commensal microbes and fermentation starter cultures. Microbial biofilm ecosystems also form on the surfaces of processing equipment. The interactions among microbes and between microbes and various surfaces play an important role in the persistence and the prevalence of these microbes in the food environment. Diversity of food matrices adds complexity to and directly shapes the composition of the microbiota in these ecosystems. Proper identification and quantification of microbes, evaluation of potential risks in the food ecosystems, and characterization of the physical and molecular events involved in ecosystems, including biofilm development, are among the primary tasks for food microbiologists. The objectives of this study are to develop a rapid detection system for foodborne microorganisms using molecular approaches, to characterize component(s) involved in biofilm development, and to examine the contribution of commensal organisms in ecosystem development and horizontal gene transfer.

ISBN: 9780542421600Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development.
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245 1 0 $a Identification of microorganisms in food ecosystems and characterization of physical and molecular events involved in biofilm development.
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500 $a Source: Dissertation Abstracts International, Volume: 66-11, Section: B, page: 5743.
500 $a Co-Advisers: Hua Wang; Ahmed Yousef.
502 $a Thesis (Ph.D.)--The Ohio State University, 2005.
520 $a Most foods can be considered as ecosystems containing various microorganisms including pathogenic, spoilage, commensal microbes and fermentation starter cultures. Microbial biofilm ecosystems also form on the surfaces of processing equipment. The interactions among microbes and between microbes and various surfaces play an important role in the persistence and the prevalence of these microbes in the food environment. Diversity of food matrices adds complexity to and directly shapes the composition of the microbiota in these ecosystems. Proper identification and quantification of microbes, evaluation of potential risks in the food ecosystems, and characterization of the physical and molecular events involved in ecosystems, including biofilm development, are among the primary tasks for food microbiologists. The objectives of this study are to develop a rapid detection system for foodborne microorganisms using molecular approaches, to characterize component(s) involved in biofilm development, and to examine the contribution of commensal organisms in ecosystem development and horizontal gene transfer.
520 $a A real-time PCR system was developed to rapidly detect Alicyclobacillus spp. and Listeria monocytogenes in food. Detection of less than 10 bacterial cells per reaction was achieved within 4-7 hours. CluA, a surface protein related to cell clumping, was found to be an important component in the development of Lactococcus lactis biofilm. Biofilm attributes can be disseminated within the ecosystem by conjugation, and a lactococcal strain carrying the intrinsic high frequency gene transfer mechanism could increase the transfer of pAMbeta1 by 10,000-fold. The study demonstrated, for the first time, that conjugation facilitated biofilm formation in Gram-positive bacteria, and that commensal organisms not only served as a gene pool but also as enhancers facilitating horizontal gene transfer, including the dissemination of the drug resistance genes. Finally, our data showed that commensal organisms, such as Pseudomonas, enhanced mixed-culture biofilm formation involving other microorganisms such as Staphylococcus , suggesting the contribution of commensal organisms in pathogen persistence.
520 $a The rapid detection system developed in this study, has direct applications in both food industry and basic scientific research. Results from this study advanced the knowledge in biofilm development and gene transfer mechanisms.
590 $a School code: 0168.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
650 4 $a Biology, Microbiology. $3 1017734
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790 1 0 $a Yousef, Ahmed, $e advisor
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791 $a Ph.D.
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