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Mechanisms by which dopamine affects...

Goodyear-Bruch, Caryl.

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Mechanisms by which dopamine affects the diaphragm.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Mechanisms by which dopamine affects the diaphragm./
Author:	Goodyear-Bruch, Caryl.
Description:	117 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4029.
Contained By:	Dissertation Abstracts International66-08B.
Subject:	Biology, Animal Physiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3185156
ISBN:	0542267292

Mechanisms by which dopamine affects the diaphragm.
Goodyear-Bruch, Caryl.

Mechanisms by which dopamine affects the diaphragm. - 117 p.

Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4029.

Thesis (Ph.D.)--The University of Kansas, 2005.

Diaphragm fatigue is a contributor of unsuccessful weaning in Intensive Care Unit (ICU) patients on mechanical ventilation. Previous animal model studies demonstrated low dose dopamine (DA) increased diaphragm blood flow, prevented and reversed diaphragm fatigue (Pierce, Clancy, Smith-Blair, & Kraft, 2002) and decreased apoptosis (Pierce, Jegathesan et al., 2004). The purpose of this dissertation was to clarify the mechanisms by which DA affects diaphragm fatigue.

ISBN: 0542267292Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Mechanisms by which dopamine affects the diaphragm.
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100 1 $a Goodyear-Bruch, Caryl. $3 1907982
245 1 0 $a Mechanisms by which dopamine affects the diaphragm.
300 $a 117 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-08, Section: B, page: 4029.
500 $a Chair: Janet D. Pierce.
502 $a Thesis (Ph.D.)--The University of Kansas, 2005.
520 $a Diaphragm fatigue is a contributor of unsuccessful weaning in Intensive Care Unit (ICU) patients on mechanical ventilation. Previous animal model studies demonstrated low dose dopamine (DA) increased diaphragm blood flow, prevented and reversed diaphragm fatigue (Pierce, Clancy, Smith-Blair, & Kraft, 2002) and decreased apoptosis (Pierce, Jegathesan et al., 2004). The purpose of this dissertation was to clarify the mechanisms by which DA affects diaphragm fatigue.
520 $a We hypothesized DA would reduce diaphragm fatigue in an in-vitro rat model by scavenging reactive oxygen species (ROS) as quantified by apoptosis using fluorescence microscopy. A visual method of counting hue values of nuclei was compared to a computer software method. There was statistical significance in the intrarater reliability (p = 0.0001) and interrater reliability (p = 0.005) using the computer and no significance with the visual method. Thus, utilizing the computer software method provided more reliable data.
520 $a We investigated diaphragm performance and apoptosis at different oxygen (O2) concentrations. In the experiments using 95% O2, there were no differences in the number of apoptotic nuclei between the DA group (3.8 +/- 4.2%) and Kreb (KR) group (3.2 +/- 2.8%). There were differences in apoptotic nuclei between the DA and KR groups in the experiments using 21% (3.3 +/- 2.4% versus 34.9 +/- 9.4%) and 10% (7.4 +/- 2.0% versus 44.2 +/- 6.2%) O2 concentrations. After drug equilibration, there were diaphragm performance differences between the DA and KR groups in the 21% (97.4 +/- 3.8% of control versus 92.8 +/- 4.8% of control) and 10% (110.3 +/- 15.7% of control versus 72.6 +/- 15.1% of control) O2 environments. DA decreased apoptosis by both scavenging ROS and activating beta2 adrenoreceptors and augmented diaphragm performance prior to stimulation by activating muscle beta2 adrenoreceptors.
520 $a Using multi-immunoblotting, 12 of 27 proteins tracked were expressed in the diaphragm. We discovered which apoptotic proteins were expressed when work of breathing was increased and following the administration of DA. Of those 12 proteins, two were significantly different when DA was administered thus, clarifying the mechanisms by which DA prevents apoptosis in the diaphragm.
590 $a School code: 0099.
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Health Sciences, Nursing. $3 1017798
690 $a 0433
690 $a 0569
710 2 0 $a The University of Kansas. $3 1288487
773 0 $t Dissertation Abstracts International $g 66-08B.
790 1 0 $a Pierce, Janet D., $e advisor
790 $a 0099
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3185156