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The effects of retinol during in viv...

Livingston, Tracy Elizabeth.

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The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development./
Author:	Livingston, Tracy Elizabeth.
Description:	153 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0004.
Contained By:	Dissertation Abstracts International65-01B.
Subject:	Agriculture, Animal Culture and Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3119403
ISBN:	0496667998

The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development.
Livingston, Tracy Elizabeth.

The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development. - 153 p.

Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0004.

Thesis (Ph.D.)--The University of Tennessee, 2003.

Previous studies demonstrated that retinol administration to ewes, followed by natural service, resulted in embryos with improved competence to develop in vitro. In vivo studies with sheep and in vitro experiments with bovine embryos, were designed to evaluate the effects of retinol and to understand its mechanism(s) of action.

ISBN: 0496667998Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development.
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035 $a (UnM)AAI3119403
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040 $a UnM $c UnM
100 1 $a Livingston, Tracy Elizabeth. $3 1907500
245 1 4 $a The effects of retinol during in vivo and in vitro oocyte maturation and embryonic development.
300 $a 153 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-01, Section: B, page: 0004.
500 $a Major Professor: James D. Godkin.
502 $a Thesis (Ph.D.)--The University of Tennessee, 2003.
520 $a Previous studies demonstrated that retinol administration to ewes, followed by natural service, resulted in embryos with improved competence to develop in vitro. In vivo studies with sheep and in vitro experiments with bovine embryos, were designed to evaluate the effects of retinol and to understand its mechanism(s) of action.
520 $a Sheep oocytes were matured in vitro over a 24 hour period in the presence of different radiolabeled substrates. Results revealed that oxidative metabolism measured by glutamine showed no significant differences over all time periods. Pyruvate oxidation was highest early in maturation and then decreased. Glycolysis was highest at the middle time period. Differences in metabolism between oocytes from retinol-treated ewes and those from control ewes were not detected.
520 $a Varying concentrations of retinol were added either during in vitro maturation (IVM) or in vitro culture (IVC) of bovine oocyte and embryos, respectively. Our results demonstrated that 5muM retinol supplementation during IVM tended to improve embryonic development measured by the rate of blastocyst development. This concentration proved even more beneficial if the control blastocyst rate was below 20%. Furthermore, 10muM retinol appeared detrimental during IVC but not during IVM.
520 $a Next, we evaluated the effects of 5muM retinol and 100muM cysteamine on bovine oocyte glutathione content. In addition, we investigated the combinatorial effects of retinol and cysteamine on in vitro bovine embryonic development. We did not observe an increase in glutathione levels in bovine oocytes treated with retinol. However, in the presence of cysteamine bovine oocytes exhibited an increase in glutathione content. Retinol and cysteamine treatment during IVM and IVC increased bovine blastocyst development, which may indicate that retinol is increasing the utilization or uptake of cysteine from the medium.
520 $a Next, we evaluated the role of exogenous retinol supplementation to superovulated ewes on the glutathione content of mature oocytes collected from the oviducts. Our results did not reveal differences in glutathione content of oocytes from retinol-treated ewes versus those from control ewes. Antioxidant transcripts encoding for Mn-SOD, Cu-Zn SOD, GS, and GSTp, were detected in ovine oocytes matured in vivo. However, retinol did not appear to impact the expression levels of these transcripts.
590 $a School code: 0226.
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Cell. $3 1017686
690 $a 0475
690 $a 0307
690 $a 0379
710 2 0 $a The University of Tennessee. $3 1022026
773 0 $t Dissertation Abstracts International $g 65-01B.
790 1 0 $a Godkin, James D., $e advisor
790 $a 0226
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3119403