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Brain-derived neurotrophic factor in...

Liang, Fong-Qi.

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Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications./
Author:	Liang, Fong-Qi.
Description:	133 p.
Notes:	Source: Dissertation Abstracts International, Volume: 59-12, Section: B, page: 6212.
Contained By:	Dissertation Abstracts International59-12B.
Subject:	Biology, Neuroscience. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9915270
ISBN:	0599139900

Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications.
Liang, Fong-Qi.

Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications. - 133 p.

Source: Dissertation Abstracts International, Volume: 59-12, Section: B, page: 6212.

Thesis (Ph.D.)--Texas A&M University, 1998.

Photic entrainment of mammalian circadian rhythms occurs because the pacemaker in the suprachiasmatic nuclei (SCN) is endowed with a rhythmic sensitivity to photic input conveyed by the retinohypothalamic tract. The present studies were designed to determine whether BDNF plays a role in the regulation of the circadian sensitivity of SCN pacemaker to light. The rat SCN were found to express the mature BDNF peptide and mRNA by Western blotting and RT-PCR analysis, respectively. BDNF immunoreactivity and hybridization signal for its mRNA were co-entensively localized within a number of cells throughout the rostrocaudal axis of the SCN. Immunostaining for TrkB the tyrosine kinase receptor that bind BDNF, was also evident in the SCN within terminals or fibers predominantly located along the SCN/optic chiasm interface and within scattered perikarya near the medial border of each nucleus. Combined in situ hybridization and immunocytochemical analysis revealed that BDNF mRNA-expressing cells within the ventrolateral SCN were often closely apposed to TrkB-positive fibers extending from the optic chiasm. BDNF expression in the SCN oscillated in a circadian fashion, with mRNA and protein reaching peak values during the early subjective day and the subjective night, respectively. Similar to the SCN in vivo, immortalized SCN cells also exhibited robust circadian oscillations in BDNF and NT-3 content. In view of BDNF involvement in the modulation of synaptic transmission in other brain regions, the present study also examined whether altered expression of BDNF or blockade of its action in the SCN would affect circadian pacemaker responses to light. Administration of exogenous BDNF into the SCN enabled light to induce phase advances at CT 6, whereas deficits in BDNF expression in the SCN of heterozygous BDNF knock-out mice significantly abated light-induced phase advances at CT 22. SCN infusion of K-252a, a tyrosine kinase inhibitor, blocked or reduced both the phase-delaying and advancing effects of light. Collectively, these findings suggest that BDNF-mediated signaling may play a critical role in the circadian regulation of SCN pacemaker sensitivity to light.

ISBN: 0599139900Subjects--Topical Terms:

1017680
Biology, Neuroscience.

Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications.
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100 1 $a Liang, Fong-Qi. $3 1907167
245 1 0 $a Brain-derived neurotrophic factor in the rat suprachiasmatic nucleus: Expression and functional implications.
300 $a 133 p.
500 $a Source: Dissertation Abstracts International, Volume: 59-12, Section: B, page: 6212.
500 $a Chair: David J. Earnest.
502 $a Thesis (Ph.D.)--Texas A&M University, 1998.
520 $a Photic entrainment of mammalian circadian rhythms occurs because the pacemaker in the suprachiasmatic nuclei (SCN) is endowed with a rhythmic sensitivity to photic input conveyed by the retinohypothalamic tract. The present studies were designed to determine whether BDNF plays a role in the regulation of the circadian sensitivity of SCN pacemaker to light. The rat SCN were found to express the mature BDNF peptide and mRNA by Western blotting and RT-PCR analysis, respectively. BDNF immunoreactivity and hybridization signal for its mRNA were co-entensively localized within a number of cells throughout the rostrocaudal axis of the SCN. Immunostaining for TrkB the tyrosine kinase receptor that bind BDNF, was also evident in the SCN within terminals or fibers predominantly located along the SCN/optic chiasm interface and within scattered perikarya near the medial border of each nucleus. Combined in situ hybridization and immunocytochemical analysis revealed that BDNF mRNA-expressing cells within the ventrolateral SCN were often closely apposed to TrkB-positive fibers extending from the optic chiasm. BDNF expression in the SCN oscillated in a circadian fashion, with mRNA and protein reaching peak values during the early subjective day and the subjective night, respectively. Similar to the SCN in vivo, immortalized SCN cells also exhibited robust circadian oscillations in BDNF and NT-3 content. In view of BDNF involvement in the modulation of synaptic transmission in other brain regions, the present study also examined whether altered expression of BDNF or blockade of its action in the SCN would affect circadian pacemaker responses to light. Administration of exogenous BDNF into the SCN enabled light to induce phase advances at CT 6, whereas deficits in BDNF expression in the SCN of heterozygous BDNF knock-out mice significantly abated light-induced phase advances at CT 22. SCN infusion of K-252a, a tyrosine kinase inhibitor, blocked or reduced both the phase-delaying and advancing effects of light. Collectively, these findings suggest that BDNF-mediated signaling may play a critical role in the circadian regulation of SCN pacemaker sensitivity to light.
590 $a School code: 0803.
650 4 $a Biology, Neuroscience. $3 1017680
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790 $a 0803
791 $a Ph.D.
792 $a 1998
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9915270