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Activity and the circadian system in...

Marchant, Elliott Gordon.

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Activity and the circadian system in the C57Bl/6J mouse.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Activity and the circadian system in the C57Bl/6J mouse./
作者:	Marchant, Elliott Gordon.
面頁冊數:	142 p.
附註:	Source: Dissertation Abstracts International, Volume: 58-12, Section: B, page: 6329.
Contained By:	Dissertation Abstracts International58-12B.
標題:	Biology, Animal Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ24334
ISBN:	0612243346

Activity and the circadian system in the C57Bl/6J mouse.
Marchant, Elliott Gordon.

Activity and the circadian system in the C57Bl/6J mouse. - 142 p.

Source: Dissertation Abstracts International, Volume: 58-12, Section: B, page: 6329.

Thesis (Ph.D.)--Simon Fraser University (Canada), 1997.

This thesis first examined two methods of studying nonphotic effects on the phase and period of circadian rhythms in C57Bl/6j (C57) mice and, second, investigated the neural source of nonphotic input to the suprachiasmatic nucleus (SCN) circadian pacemaker. Experiment 1 examined the effects of the opioid agonist morphine on free-running circadian rhythms in C57 mice. Acute injections of morphine induced locomotor activity and wheel running for several hours, and phase shifted free-running rhythms. The phase-response curve (PRC) was similar to that for locomotor activity alone, and shifts were blocked by preventing activity. Experiment 2 established that forced treadmill (TM) running can reliably entrain free-running rhythms. This method provides better control over activity intensity and duration, and was adopted for further studies of neural mechanism. Prior evidence suggested that the intergeniculate leaflet (IGL), via neuropeptide Y (NPY), and the raphe nucleus (RN), via serotonin(5HT), may play a role in conveying non-photic inputs to the pacemaker. Experiment 3 examined the effects of serotonin depletion on entrainment to scheduled TM activity. Mice entrained to scheduled TM activity were fed a trytophan free-diet, which reduces 5HT synthesis. Circadian rhythmicity was strongly attenuated within 3 days, and independent effects on entrainment could thus not be determined with confidence. Experiment 5 examined the effects of radiofrequency IGL ablations and neurotoxin induced 5-HT depletion on entrainment to TM activity. Lesions were verified by NPY and 5-HT immunocytochemistry. Mice with complete IGL or 5HT ablations failed to entrain to regular scheduled TM running. Complete 5-HT ablated mice exhibited modulations of tau, whereas complete IGLx mice showed little or no modulation of tau in response to forced TM activity. Both the IGL and the RN inputs to the SCN appear to be necessary for entrainment to 24 h schedules of forced running, but the modulations of tau evident in the mice with complete 5HT lesions suggests that they might entrain if the period of the forced running schedule matched tau more closely.

ISBN: 0612243346Subjects--Topical Terms:

1017835
Biology, Animal Physiology.

Activity and the circadian system in the C57Bl/6J mouse.
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