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Circadian control of gene expression...

Van Gelder, Russell Neil.

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Circadian control of gene expression in Drosophila melanogaster and Mus musculus.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Circadian control of gene expression in Drosophila melanogaster and Mus musculus./
Author:	Van Gelder, Russell Neil.
Description:	225 p.
Notes:	Source: Dissertation Abstracts International, Volume: 55-10, Section: B, page: 4269.
Contained By:	Dissertation Abstracts International55-10B.
Subject:	Biology, Neuroscience. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9508460

Circadian control of gene expression in Drosophila melanogaster and Mus musculus.
Van Gelder, Russell Neil.

Circadian control of gene expression in Drosophila melanogaster and Mus musculus. - 225 p.

Source: Dissertation Abstracts International, Volume: 55-10, Section: B, page: 4269.

Thesis (Ph.D.)--Stanford University, 1994.

Circadian rhythms are the nearly-24 hour rhythms of physiology and behavior that are a ubiquitous feature of eukaryotic life. The period (per) gene of Drosophila melanogaster is necessary for the expression of circadian rhythms in this species. Recent studies have suggested that the period gene functions in an autofeedback loop whereby the Per protein modulate its own mRNA expression in a circadian fashion; such a feedback loop may underlie the mechanism of the central circadian pacemaker.Subjects--Topical Terms:

1017680
Biology, Neuroscience.

Circadian control of gene expression in Drosophila melanogaster and Mus musculus.
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008 130610s1994 eng d
035 $a (UnM)AAI9508460
035 $a AAI9508460
040 $a UnM $c UnM
100 1 $a Van Gelder, Russell Neil. $3 1907138
245 1 0 $a Circadian control of gene expression in Drosophila melanogaster and Mus musculus.
300 $a 225 p.
500 $a Source: Dissertation Abstracts International, Volume: 55-10, Section: B, page: 4269.
500 $a Adviser: William C. Dement.
502 $a Thesis (Ph.D.)--Stanford University, 1994.
520 $a Circadian rhythms are the nearly-24 hour rhythms of physiology and behavior that are a ubiquitous feature of eukaryotic life. The period (per) gene of Drosophila melanogaster is necessary for the expression of circadian rhythms in this species. Recent studies have suggested that the period gene functions in an autofeedback loop whereby the Per protein modulate its own mRNA expression in a circadian fashion; such a feedback loop may underlie the mechanism of the central circadian pacemaker.
520 $a In this dissertation, I explore several facets of the circadian control of gene expression in Drosophila and the mouse Mus musculus. A screen for circadianly expressed mRNAs from a collection of 280 cDNAs known to be expressed in the adult fly head but not the early fly embryo is described. From this collection, 20 cDNAs were identified as undergoing diurnal rhythms of greater than two-fold in expression in fly heads; seventeen of these genes are maximally expressed during the dark period. These genes are called the DREGs, for Drosophila rhythmically expressed genes. Further characterization of four of these genes is presented. Dregs-1, 2, and 3 are maximally expressed during daytime. Dreg-1 encodes alcohol dehydrogenase, while the other two are novel genes. None are strictly dependent on period for their rhythmicity, but all are dependent on an external time cue. Dreg-5 is a novel nocturnally expressed mRNA fully dependent on period for its rhythmic expression and expressed in phase with per mRNA. Dreg-5 protein is also expressed with a circadian rhythm.
520 $a A model is presented demonstrating that the period autofeedback cycle is sufficient to explain many of the formal properties observed in circadian rhythms. Additionally, two new techniques for the performance of mammalian molecular chronobiological experiments are presented: one technique allows the long-term performance of circadian sleep-wake recordings in mice, while the second technique allows the amplification of large amounts of cDNA from small amounts of starting total RNA. These techniques are used in tandem to generate a cDNA library from the suprachiasmatic nucleus. This library is screened differentially to identify a diurnally expressed LINE element in the mammalian SCN.
590 $a School code: 0212.
650 4 $a Biology, Neuroscience. $3 1017680
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
690 $a 0317
690 $a 0307
690 $a 0369
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 55-10B.
790 1 0 $a Dement, William C., $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 1994
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9508460