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Colloidal gold as molecular capture ...

Kirk, John Samuel, VIII.

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Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis./
Author:	Kirk, John Samuel, VIII.
Description:	118 p.
Notes:	Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0236.
Contained By:	Dissertation Abstracts International67-01B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3202115
ISBN:	0542502046

Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis.
Kirk, John Samuel, VIII.

Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis. - 118 p.

Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0236.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.

Sample handling is of great import to a number of applications where the target analyte is mass-limited (e.g. biochemical warfare detection or single-cell biochemical research). Particularly troublesome is sample loss through interfacial adsorption, along with the complexity of purifying, concentrating, and identifying the target molecule. One strategy that can be used to circumvent these problems is to arrange for the adsorption to occur on the surface of a carrier, which can subsequently be manipulated.

ISBN: 0542502046Subjects--Topical Terms:

586156
Chemistry, Analytical.

Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis.
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035 $a AAI3202115
040 $a UnM $c UnM
100 1 $a Kirk, John Samuel, VIII. $3 1906961
245 1 0 $a Colloidal gold as molecular capture devices in hybrid nano/microfluidic systems for mass-limited sample analysis.
300 $a 118 p.
500 $a Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0236.
500 $a Adviser: Paul W. Bohn.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2005.
520 $a Sample handling is of great import to a number of applications where the target analyte is mass-limited (e.g. biochemical warfare detection or single-cell biochemical research). Particularly troublesome is sample loss through interfacial adsorption, along with the complexity of purifying, concentrating, and identifying the target molecule. One strategy that can be used to circumvent these problems is to arrange for the adsorption to occur on the surface of a carrier, which can subsequently be manipulated.
520 $a This thesis describes the development of colloidal gold as a means of efficiently sequestering analytes from solution, enabling easy purification, concentration, and identification of the captured molecule. Model targets used were the organomercaptan, 5-((2-(and-3)-S-(acetylmercapto)-succinoyl)amino) fluorescein (SAMSA) and the peptide, H2N-Cys-Lys-Trp-Ala-Lys-Trp-Ala-Trp-CO 2NH2 (CKWAKWAK). In order to circumvent difficulties from quenching of fluorescent molecules close to the surface of the metallic particle, a difference fluorimetry method was developed. Using this method, the loading capacity of the colloidal gold was determined in surface adsorption isotherms and fit well to a Langmuir model. The adsorbate/colloid conjugates were characterized by UV-visible spectroscopy and transmission electron microscopy (TEM) to determine their stability in solution. The aggregation state of these conjugates is a good gauge of the ability to manipulate the conjugates with minimal sample loss through interfacial adsorption. The surface-bound analyte was also detected directly through matrix-assisted laser desorption and ionization mass spectrometry (MALDI-MS), demonstrating the viability of using colloidal gold to avoid typical mass-limited sample analysis difficulties.
520 $a Analyte capture by the colloidal gold was demonstrated in a hybrid nano/microfluidic device designed for sequential fluid manipulations in three dimensions. A nanocapillary array membrane (NCAM) was utilized to enable efficient mixing of the gold colloid and the adsorbate in-device. Fluorescence microscopy was used to monitor fluorescence quenching during monolayer self-assembly of analytes on the colloidal gold surface. The kinetics of the heterogeneous reaction was fit using a second order reversible kinetics model and the adsorption rate constant was compared to reported values for thiols on planar gold substrates.
590 $a School code: 0090.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a University of Illinois at Urbana-Champaign. $3 626646
773 0 $t Dissertation Abstracts International $g 67-01B.
790 1 0 $a Bohn, Paul W., $e advisor
790 $a 0090
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3202115