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Studies on the expression and functi...

Blaylock, Matthew Lantz.

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Studies on the expression and function of uncoupling protein 3.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Studies on the expression and function of uncoupling protein 3./
Author:	Blaylock, Matthew Lantz.
Description:	91 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-05, Section: B, page: 2348.
Contained By:	Dissertation Abstracts International65-05B.
Subject:	Health Sciences, Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3132291
ISBN:	0496795317

Studies on the expression and function of uncoupling protein 3.
Blaylock, Matthew Lantz.

Studies on the expression and function of uncoupling protein 3. - 91 p.

Source: Dissertation Abstracts International, Volume: 65-05, Section: B, page: 2348.

Thesis (Ph.D.)--The University of Alabama at Birmingham, 2003.

Objective. The purpose of this work was to study the expression and function of uncoupling protein (UCP) 3. First, this work will examine the association between Ucp3 expression and energy expenditure. Second, the relationship between free-fatty acids (FFA) and Ucp3 expression will be explored. Last, a novel hypothesis will be tested that proposes UCP3 functions, along with the glycerol-3-phosphate (G-3-P) shuttle, to allow the mitochondrial reoxidation of cytosolically generated reduced nicotinamide adenine dinucleotide.

ISBN: 0496795317Subjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Studies on the expression and function of uncoupling protein 3.
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020 $a 0496795317
035 $a (UnM)AAI3132291
035 $a AAI3132291
040 $a UnM $c UnM
100 1 $a Blaylock, Matthew Lantz. $3 1905990
245 1 0 $a Studies on the expression and function of uncoupling protein 3.
300 $a 91 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-05, Section: B, page: 2348.
500 $a Chair: Timothy Ralph Nagy.
502 $a Thesis (Ph.D.)--The University of Alabama at Birmingham, 2003.
520 $a Objective. The purpose of this work was to study the expression and function of uncoupling protein (UCP) 3. First, this work will examine the association between Ucp3 expression and energy expenditure. Second, the relationship between free-fatty acids (FFA) and Ucp3 expression will be explored. Last, a novel hypothesis will be tested that proposes UCP3 functions, along with the glycerol-3-phosphate (G-3-P) shuttle, to allow the mitochondrial reoxidation of cytosolically generated reduced nicotinamide adenine dinucleotide.
520 $a Research methods and procedures. The methods and procedures of this work include polymerase chain reaction-based cloning of the lemming Ucp3 gene, Northern blot analyses, isolation of muscle mitochondria and measurements of mitochondrial oxygen consumption, body composition measurements, blood metabolite and hormone analyses, and a mouse swimming protocol.
520 $a Results. The results of the lemming experiments showed that photoperiod manipulation alters Ucp3 expression but does not result in differences in mitochondrial coupling as assessed by measuring the respiratory coupling ratio. Exposing lemming to cold for 10 days decreases Ucp3 expression, whereas fasting increases Ucp3 expression. The Ucp3 expression in Zucker fatty rats was either significantly lower or not different from wild-type (WT) controls, depending on the age of the animals. There was no difference in Ucp3 expression between peroxisome proliferator-activated receptor-alpha (PPAR-alpha) knock-out (KO) mice and WT controls under fed or fasted conditions. UCP3 KO mice swam for a significantly shorter duration than WT controls did, and the rate of oxygen consumption of mitochondria isolated from UCP3 KO mice was significantly lower than from WT controls when G-3-P was used as a substrate.
520 $a Summary. The results of the lemming work suggest that Ucp3 expression is not directly related to changes in resting energy expenditure or to thermogenic requirements in the collared lemming. The Zucker rat data suggest that sustained, high plasma FFA levels do not result in high Ucp3 expression. The PPAR-alpha mouse work demonstrates that PPAR-alpha is not required for increased plasma FFA levels to increase Ucp3 expression. The UCP3 KO mouse work showed that UCP3 is required for the normal oxidation of G-3-P as a substrate. The disruption in G-3-P oxidation may be in part responsible for the decrease in swim time seen in the UCP3 KO mouse.
590 $a School code: 0005.
650 4 $a Health Sciences, Nutrition. $3 1017801
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0570
690 $a 0307
690 $a 0487
710 2 0 $a The University of Alabama at Birmingham. $3 1019443
773 0 $t Dissertation Abstracts International $g 65-05B.
790 1 0 $a Nagy, Timothy Ralph, $e advisor
790 $a 0005
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3132291