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Genechip microarray analyses of infl...

Bauer, Dina Agnes.

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Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats./
Author:	Bauer, Dina Agnes.
Description:	196 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1786.
Contained By:	Dissertation Abstracts International65-04B.
Subject:	Health Sciences, Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3130324
ISBN:	0496775731

Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats.
Bauer, Dina Agnes.

Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats. - 196 p.

Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1786.

Thesis (Ph.D.)--Wayne State University, 2004.

Hypertension and hypercholesterolemia may function as predisposing agents to cerebrovascular accidents. Evidence suggests that inflammation may play a role in this process, but the molecular controls involved remain elusive. The objectives of this study were (1) To determine the effect of supplementing cholesterol and oxidized cholesterol on high-density lipoprotein cholesterol levels (HDL-C) and on the etiology of hypertension and stroke in male spontaneously hypertensive stroke prone rats (SHRSP), (2) To determine how genes related to inflammation, hypertension and stroke are expressed in response to cholesterol and oxidized cholesterol over time. Sixty-two weanling male SHRSP rats were divided into 4--6 animals/group and fed chow diets supplemented with either cholesterol (CHOL) (2%, wt/wt) or oxidized cholesterol (OXI) (0.2% wt/wt) for 90 days. Blood pressure was assessed on days 30, 60, and 90 by the femoral artery cannulation technique. GeneChipRTM arrays were utilized to interrogate the expression of 15,866 known genes to determine the gene expression profile altered by CHOL/OXI treatment. The results showed that hypertension is inflammatory, but in the presence of dietary cholesterol/oxidized cholesterol the effects are exacerbated. Elevated pulse pressure, hepatic CRP mRNA expression, and VCAM-1 mRNA expression in the aorta with decreased plasma HDL-C are in agreement with the above observation. Furthermore, the 60-day cDNA SHRSP aorta data suggests that both cholesterol and oxidized cholesterol promote inflammation. Vascular dysfunction may, in part, be mediated by oxidative changes within the blood vessel. In addition, cholesterol and oxidized cholesterol may affect blood pressure regulation, inducing shear stress and subsequent endothelial damage. It is evident from this study that developing therapeutic agents aimed at targeting inflammatory processes may reduce the morbidity and mortality attributed to cerebrovascular accidents.

ISBN: 0496775731Subjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats.
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245 1 0 $a Genechip microarray analyses of inflammation related gene expression in response to hypertension and cholesterol/oxidized cholesterol supplementation in spontaneously hypertensive stroke prone rats.
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500 $a Source: Dissertation Abstracts International, Volume: 65-04, Section: B, page: 1786.
500 $a Adviser: Thomas V. Fungwe.
502 $a Thesis (Ph.D.)--Wayne State University, 2004.
520 $a Hypertension and hypercholesterolemia may function as predisposing agents to cerebrovascular accidents. Evidence suggests that inflammation may play a role in this process, but the molecular controls involved remain elusive. The objectives of this study were (1) To determine the effect of supplementing cholesterol and oxidized cholesterol on high-density lipoprotein cholesterol levels (HDL-C) and on the etiology of hypertension and stroke in male spontaneously hypertensive stroke prone rats (SHRSP), (2) To determine how genes related to inflammation, hypertension and stroke are expressed in response to cholesterol and oxidized cholesterol over time. Sixty-two weanling male SHRSP rats were divided into 4--6 animals/group and fed chow diets supplemented with either cholesterol (CHOL) (2%, wt/wt) or oxidized cholesterol (OXI) (0.2% wt/wt) for 90 days. Blood pressure was assessed on days 30, 60, and 90 by the femoral artery cannulation technique. GeneChipRTM arrays were utilized to interrogate the expression of 15,866 known genes to determine the gene expression profile altered by CHOL/OXI treatment. The results showed that hypertension is inflammatory, but in the presence of dietary cholesterol/oxidized cholesterol the effects are exacerbated. Elevated pulse pressure, hepatic CRP mRNA expression, and VCAM-1 mRNA expression in the aorta with decreased plasma HDL-C are in agreement with the above observation. Furthermore, the 60-day cDNA SHRSP aorta data suggests that both cholesterol and oxidized cholesterol promote inflammation. Vascular dysfunction may, in part, be mediated by oxidative changes within the blood vessel. In addition, cholesterol and oxidized cholesterol may affect blood pressure regulation, inducing shear stress and subsequent endothelial damage. It is evident from this study that developing therapeutic agents aimed at targeting inflammatory processes may reduce the morbidity and mortality attributed to cerebrovascular accidents.
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