東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Sensitivity enhancement in liquid ch...

Gao, Songmei.

Linked to FindBook

Google Book

Amazon

博客來

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives./
Author:	Gao, Songmei.
Description:	186 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2598.
Contained By:	Dissertation Abstracts International66-05B.
Subject:	Chemistry, Pharmaceutical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3177621
ISBN:	0542171724

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives.
Gao, Songmei.

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives. - 186 p.

Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2598.

Thesis (Ph.D.)--Virginia Commonwealth University, 2005.

High performance liquid chromatography with atmospheric pressure ionization (API) mass spectrometry has been essential to a large number of quantitative analytical applications for a variety of compounds. Poor detection sensitivity however is a problem observed for a number of analytes because detection sensitivity can be affected by many factors. The two most critical factors are the chemical and physical properties of the analyte and the composition of the mobile phase. In order to address these critical factors which may lead to poor sensitivity, either the structure of the analyte must be modified or the mobile phase composition optimized.

ISBN: 0542171724Subjects--Topical Terms:

550957
Chemistry, Pharmaceutical.

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives.
LDR:03077nmm 2200301 4500 001 1815698
005 20060710080809.5
008 130610s2005 eng d
020 $a 0542171724
035 $a (UnM)AAI3177621
035 $a AAI3177621
040 $a UnM $c UnM
100 1 $a Gao, Songmei. $3 1905111
245 1 0 $a Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives.
300 $a 186 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2598.
500 $a Director: H. Thomas Karnes.
502 $a Thesis (Ph.D.)--Virginia Commonwealth University, 2005.
520 $a High performance liquid chromatography with atmospheric pressure ionization (API) mass spectrometry has been essential to a large number of quantitative analytical applications for a variety of compounds. Poor detection sensitivity however is a problem observed for a number of analytes because detection sensitivity can be affected by many factors. The two most critical factors are the chemical and physical properties of the analyte and the composition of the mobile phase. In order to address these critical factors which may lead to poor sensitivity, either the structure of the analyte must be modified or the mobile phase composition optimized.
520 $a This study investigated the enhancement of sensitivity for small molecules in biomedical analyses using atmospheric-pressure chemical ionization (APCI) following derivatization, as well as improvement of sensitivity in the electrospray ionization (ESI) mode using mobile-phase additives (alkylamines and ion-pair reagents). Propylene glycol (PG) was selected as the model compound for the former method. After introduction of a benzoyl moiety to increase the proton affinity of PG, a relatively high sensitivity of detection was achieved. Paclitaxel and docetaxel served as model compounds for the alkylamine additives method. Sensitivity and precision of detection were improved approximately five-fold through the formation of one predominant alkylamine adduct while suppressing the formation of other multiple molecule adduct ions. The volatile ion-pair reagents perfluorinated carboxylic acids were used to increase the retention of polar ionizable compounds. The sensitivity of detection was increased after matrix suppression was decreased by separating the analyte from the polar endogenous co-eluting substances. The model analyses evaluated were 1-methylnicotinamide (MNA), N-methyl 2-pyridone 5-carboxamide (2PY), phenylephrine.
520 $a Each method was validated in order to demonstrate its utility, and the mechanisms of each approach were investigated with a view to expanding their potential use in future applications.
590 $a School code: 2383.
650 4 $a Chemistry, Pharmaceutical. $3 550957
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0491
690 $a 0486
710 2 0 $a Virginia Commonwealth University. $3 1018010
773 0 $t Dissertation Abstracts International $g 66-05B.
790 1 0 $a Karnes, H. Thomas, $e advisor
790 $a 2383
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3177621