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The effects of plasmid DNA and immun...

Uwiera, Richard Robert Ernest.

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The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes./
Author:	Uwiera, Richard Robert Ernest.
Description:	235 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3203.
Contained By:	Dissertation Abstracts International64-07B.
Subject:	Health Sciences, Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ80942
ISBN:	0612809420

The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes.
Uwiera, Richard Robert Ernest.

The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes. - 235 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3203.

Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2002.

This Ph.D. dissertation examined the effects of bacterial DNA on immune surveillance in ovine lymph nodes (LN). The first study identified long-term changes in lymph nodes following exposure to plasmid DNA. Treated lymph nodes were heavier and had larger medullary areas. Furthermore, medullary cord thickness and medullary sinus width as well as germinal center size and number were increased in plasmid treated lymph nodes.

ISBN: 0612809420Subjects--Topical Terms:

1017854
Health Sciences, Pathology.

The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes.
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035 $a (UnM)AAINQ80942
035 $a AAINQ80942
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100 1 $a Uwiera, Richard Robert Ernest. $3 1904658
245 1 4 $a The effects of plasmid DNA and immunostimulatory CpG motifs on immune surveillance in sheep lymph nodes.
300 $a 235 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3203.
500 $a Advisers: D. Middleton; P. Griebel.
502 $a Thesis (Ph.D.)--The University of Saskatchewan (Canada), 2002.
520 $a This Ph.D. dissertation examined the effects of bacterial DNA on immune surveillance in ovine lymph nodes (LN). The first study identified long-term changes in lymph nodes following exposure to plasmid DNA. Treated lymph nodes were heavier and had larger medullary areas. Furthermore, medullary cord thickness and medullary sinus width as well as germinal center size and number were increased in plasmid treated lymph nodes.
520 $a To determine whether bacterial DNA altered cell trafficking through lymph nodes, the efferent lymphatic of the prescapular LN of sheep was cannulated and cells were collected. Intradermal injection of as little as 4 mug of plasmid DNA expressing the green fluorescent protein of jellyfish (eGFP) caused a marked increase in the cell trafficking through the prescapular lymph node. A dose-dependent facet existed for this response, as the increase in cell trafficking response persisted longer with 40 mug or 400 mug of plasmid-eGFP than with 4 mug. This increased cell trafficking was independent of green fluorescent protein expression as both pCAN1-eGFP and pCAN1 induced similar responses.
520 $a Increased cell traffic induced by bacterial DNA was further characterized by determining whether bacterial DNA form was critical for this response. Treatment with intact plasmid (circular) DNA induced the greatest increase in cell traffic. In contrast, when plasmid DNA was digested with restriction enzymes into linear fragments of DNA, then cell trafficking was not significantly increased.
520 $a Numerous studies have shown that immunostimulatory guanosine-cytosine (CpG) motifs activate cells in vitro and can improve protective immune responses in vivo. Therefore we tested the hypothesis that the presence of immunostimulatory CpG motifs within plasmids was responsible for altering cell trafficking. It was shown that plasmids with added immunostimulatory CpG sequences affected cell trafficking in a dose-dependent manner. The injection of 40 mug of pBISIA-88 caused an increase in cell trafficking while injecting 400 mug pBISIA-88 failed to increase cell flow above control levels.
520 $a Analysis of cell populations collected in efferent lymph gives insight into treatment effects on immune surveillance. This is well-studied following treatment with antigen and in general, antigen induces a selective movement of cells into lymph that occurs 24 hours post-treatment. (Abstract shortened by UMI.)
590 $a School code: 0780.
650 4 $a Health Sciences, Pathology. $3 1017854
650 4 $a Health Sciences, Immunology. $3 1017716
650 4 $a Biology, Veterinary Science. $3 1021733
690 $a 0571
690 $a 0982
690 $a 0778
710 2 0 $a The University of Saskatchewan (Canada). $3 1025078
773 0 $t Dissertation Abstracts International $g 64-07B.
790 1 0 $a Middleton, D., $e advisor
790 1 0 $a Griebel, P., $e advisor
790 $a 0780
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NQ80942