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Nodal-related signaling during zebra...

Erter, Caroline Elizabeth.

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Nodal-related signaling during zebrafish embryogenesis.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Nodal-related signaling during zebrafish embryogenesis./
作者:	Erter, Caroline Elizabeth.
面頁冊數:	165 p.
附註:	Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5664.
Contained By:	Dissertation Abstracts International61-11B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9996247
ISBN:	049304325X

Nodal-related signaling during zebrafish embryogenesis.
Erter, Caroline Elizabeth.

Nodal-related signaling during zebrafish embryogenesis. - 165 p.

Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5664.

Thesis (Ph.D.)--Vanderbilt University, 2000.

I describe the Nodal-related family of signaling molecules that have been implicated in mesodermal and neural patterning, and left-right asymmetry, in mouse, frog, and chicken embryos. My studies focus on the isolation and characterization of one zebrafish <italic>nodal</italic> orthologue, <italic> squint</italic> (<italic>sqt</italic>). The early expression pattern of <italic> sqt</italic> begins dorsally in both the embryonic and extraembryonic tissue. Overexpression of <italic>sqt</italic> in whole embryos expands or duplicates axial cell fates. Furthermore, Sqt overexpression, exclusively in the extraembryonic yolk syncytial layer (YSL), causes broadened or duplicated dorsal mesodermal marker gene expression in the overlying blastoderm. Additionally, the inductive properties of Sqt are compared to another fish Nodal-related ligand, Cyc. Sqt is an efficient inducer of organizer-type mesendodermal markers, while Cyc induces both mesodermal and neural fates.

ISBN: 049304325XSubjects--Topical Terms:

1017686
Biology, Cell.

Nodal-related signaling during zebrafish embryogenesis.
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300 $a 165 p.
500 $a Source: Dissertation Abstracts International, Volume: 61-11, Section: B, page: 5664.
500 $a Director: Christopher V. E. Wright.
502 $a Thesis (Ph.D.)--Vanderbilt University, 2000.
520 $a I describe the Nodal-related family of signaling molecules that have been implicated in mesodermal and neural patterning, and left-right asymmetry, in mouse, frog, and chicken embryos. My studies focus on the isolation and characterization of one zebrafish <italic>nodal</italic> orthologue, <italic> squint</italic> (<italic>sqt</italic>). The early expression pattern of <italic> sqt</italic> begins dorsally in both the embryonic and extraembryonic tissue. Overexpression of <italic>sqt</italic> in whole embryos expands or duplicates axial cell fates. Furthermore, Sqt overexpression, exclusively in the extraembryonic yolk syncytial layer (YSL), causes broadened or duplicated dorsal mesodermal marker gene expression in the overlying blastoderm. Additionally, the inductive properties of Sqt are compared to another fish Nodal-related ligand, Cyc. Sqt is an efficient inducer of organizer-type mesendodermal markers, while Cyc induces both mesodermal and neural fates.
520 $a Additionally, using <italic>cyc</italic>;<italic>sqt</italic> double mutants, I describe the process of neural posteriorization/transformation, which is essential for anterior-posterior (A-P) regionalization of the vertebrate central nervous system (CNS). I explore the molecular identity of the influence present in lateral mesodermal precursors that are maintained in Nodal-deficient embryos, which exerts a posteriorizing effect on anterior neural tissue. Employing a variety of genetic situations that vary the extent of mesendodermal precursor formation, I find that expression of <italic>wnt8</italic>, a previously implicated neural transformer, is necessary for posteriorization. Moreover, specific inhibition of <italic>wnt8</italic> abrogates posteriorization <italic>in vivo.</italic> My findings provide strong evidence that Wnt8 acts in lateral mesendodermal precursors, independent of Sqt and Cyc, as an essential component of the neural transformation process.
520 $a Lastly, I define <italic>cis</italic>-acting regulatory regions within the <italic>sqt</italic> upstream region that drive GFP reporter gene expression in the endogenous <italic>sqt</italic> expression domains in transient transgenic assays. A −9 kb segment upstream of the start ATG drives GFP throughout the extraembryonic yolk syncytial layer (YSL), a region of the embryo shown to induce overlying blastomeres to adopt a mesendodermal fate, and in deep and superficial cells of the marginal zone. Using standard deletion analyses, we narrowed the region mostly responsible for YSL and deep marginal blastodermal expression to a 1 kb fragment (−1.3 to −0.3 kb) upstream of the translational start site.
590 $a School code: 0242.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
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