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Wnt signaling in early zebrafish dev...

Nasevicius, Aidas.

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Wnt signaling in early zebrafish development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Wnt signaling in early zebrafish development./
Author:	Nasevicius, Aidas.
Description:	140 p.
Notes:	Source: Dissertation Abstracts International, Volume: 62-03, Section: B, page: 1202.
Contained By:	Dissertation Abstracts International62-03B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3008718
ISBN:	0493190635

Wnt signaling in early zebrafish development.
Nasevicius, Aidas.

Wnt signaling in early zebrafish development. - 140 p.

Source: Dissertation Abstracts International, Volume: 62-03, Section: B, page: 1202.

Thesis (Ph.D.)--University of Minnesota, 2001.

Zebrafish was used as a model organism to investigate the importance of Wnt signaling in early vertebrate development. Three candidate Wnt protein receptors frizzled A, frizzled C, and 10 were cloned. The frizzleds, maternally encoded zebrafish wnt8, and several other Wnt pathway components were overexpressed via synthetic mRNA microinjection. The obtained data indicated that Wnt signaling is sufficient to induce the dorsoventral axis in the early zebrafish embryo. A dominant negative form of maternally expressed frizzled A was constructed and expressed together with dominant negative forms of several wnt pathway members. The experimental results supported the notion that Wnt signaling is required for dorsal mesoderm formation in vertebrates.

ISBN: 0493190635Subjects--Topical Terms:

1017730
Biology, Genetics.

Wnt signaling in early zebrafish development.
LDR:02355nmm 2200301 4500 001 1810429
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100 1 $a Nasevicius, Aidas. $3 1900041
245 1 0 $a Wnt signaling in early zebrafish development.
300 $a 140 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-03, Section: B, page: 1202.
500 $a Adviser: Stephen C. Ekker.
502 $a Thesis (Ph.D.)--University of Minnesota, 2001.
520 $a Zebrafish was used as a model organism to investigate the importance of Wnt signaling in early vertebrate development. Three candidate Wnt protein receptors frizzled A, frizzled C, and 10 were cloned. The frizzleds, maternally encoded zebrafish wnt8, and several other Wnt pathway components were overexpressed via synthetic mRNA microinjection. The obtained data indicated that Wnt signaling is sufficient to induce the dorsoventral axis in the early zebrafish embryo. A dominant negative form of maternally expressed frizzled A was constructed and expressed together with dominant negative forms of several wnt pathway members. The experimental results supported the notion that Wnt signaling is required for dorsal mesoderm formation in vertebrates.
520 $a The possibility of functional interaction between Wnt and Notch signaling pathways was investigated. The preliminary data obtained from the overexpression studies of Notch and members of Wnt pathway indicated that Notch could be a candidate Wnt receptor.
520 $a An efficient specific gene knockdown technique was developed. The technique uses modified oligonucleotides—morpholino phosphorodiamidate oligonucleotides (morpholinos). Delivery of morpholinos into the early embryos <italic>via </italic> microinjection specifically inhibits the translation of the gene of interest. This technique was used to knockdown several genes. One of them, VEGF A, is a candidate gene for drug development for cancer treatment.
590 $a School code: 0130.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0369
690 $a 0307
710 2 0 $a University of Minnesota. $3 676231
773 0 $t Dissertation Abstracts International $g 62-03B.
790 1 0 $a Ekker, Stephen C., $e advisor
790 $a 0130
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3008718