東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Force and shape coordination in amoe...

Alonso-Latorre, Baldomero.

Linked to FindBook

Google Book

Amazon

博客來

Force and shape coordination in amoeboid cell motility.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Force and shape coordination in amoeboid cell motility./
Author:	Alonso-Latorre, Baldomero.
Description:	152 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 0128.
Contained By:	Dissertation Abstracts International72-01B.
Subject:	Biology, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3432150
ISBN:	9781124355726

Force and shape coordination in amoeboid cell motility.
Alonso-Latorre, Baldomero.

Force and shape coordination in amoeboid cell motility. - 152 p.

Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 0128.

Thesis (Ph.D.)--University of California, San Diego, 2010.

Cell motility plays an essential role in many physiological and pathological processes, yet we still lack information about the spatio-temporal coordination between regulatory biochemical processes and mechanics of cell migration. This dissertation has investigated the mechanics of amoeboid cell migration through intensive analysis of the traction forces exerted and shapes adopted by single Dictyostelium discoideum cells migrating chemotactically, focusing on wild-type (WT) and contractility-defective cells lacking either protein myosin II (mhcA-) or the myosin II essential light chains (mlcE-).

ISBN: 9781124355726Subjects--Topical Terms:

1018625
Biology, General.

Force and shape coordination in amoeboid cell motility.
LDR:03609nam 2200397 4500 001 1404619
005 20111130124100.5
008 130515s2010 ||||||||||||||||| ||eng d
020 $a 9781124355726
035 $a (UMI)AAI3432150
035 $a AAI3432150
040 $a UMI $c UMI
100 1 $a Alonso-Latorre, Baldomero. $3 1683952
245 1 0 $a Force and shape coordination in amoeboid cell motility.
300 $a 152 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: 0128.
500 $a Advisers: Juan C. Lasheras; Juan C. del Alamo.
502 $a Thesis (Ph.D.)--University of California, San Diego, 2010.
520 $a Cell motility plays an essential role in many physiological and pathological processes, yet we still lack information about the spatio-temporal coordination between regulatory biochemical processes and mechanics of cell migration. This dissertation has investigated the mechanics of amoeboid cell migration through intensive analysis of the traction forces exerted and shapes adopted by single Dictyostelium discoideum cells migrating chemotactically, focusing on wild-type (WT) and contractility-defective cells lacking either protein myosin II (mhcA-) or the myosin II essential light chains (mlcE-).
520 $a We have developed an improved traction force cytometry method to calculate cell traction stresses which considers the finite thickness of the substrate. We have shown that the strain energy exerted by locomoting cells on the substrate evolves quasi-periodically and correlates with cell length, and thus it can be used as a quantitative indicator of the cell motility cycle. The periodicity (T) of the oscillations in the traction forces correlates strongly with the average velocity of migration (V) of cells according to the hyperbolic law VT=lambda, where the constant lambda is independent of the strain analyzed and corresponds to the average distance a cell travels per cycle.
520 $a Given the quasi-periodic character of both cell length and strain energy, we have performed a phase statistical analysis to obtain a spatio-temporal representation of the canonical motility cycle divided into four phases: protrusion, contraction, retraction, and relaxation. This analysis has elucidated the role that protein myosin II plays in enhancing the kinetics of the four stages of the cycle and in controlling the spatial distribution of the traction forces regulating that process.
520 $a We have used principal component analysis to dissect the mechanics of locomotion of amoeboid cells into a reduced set of dominant components of cellular traction forces and shape changes. The dominant traction force component accounts for 40% of the strain energy performed by these cells, and its temporal evolution correlates with the quasi-periodic variations of cell length and strain energy exerted on the substrate.
520 $a Finally, we have developed two analytic assays for the calculation of cell traction stresses in configurations of interest to further understand the mechanosensing machinery of cells.
590 $a School code: 0033.
650 4 $a Biology, General. $3 1018625
650 4 $a Biophysics, Biomechanics. $3 1035342
650 4 $a Biophysics, General. $3 1019105
690 $a 0306
690 $a 0648
690 $a 0786
710 2 $a University of California, San Diego. $b Engineering Sciences (Engineering Physics). $3 1036190
773 0 $t Dissertation Abstracts International $g 72-01B.
790 1 0 $a Lasheras, Juan C., $e advisor
790 1 0 $a del Alamo, Juan C., $e advisor
790 1 0 $a Firtel, Richard A. $e committee member
790 1 0 $a Chien, Shu $e committee member
790 1 0 $a Lauga, Eric $e committee member
790 1 0 $a Marsden, Alison $e committee member
790 $a 0033
791 $a Ph.D.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3432150