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Gene activation potential of MsHsfA4...

Byczko, Helen Catherine.

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Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L./
Author:	Byczko, Helen Catherine.
Description:	90 p.
Notes:	Source: Masters Abstracts International, Volume: 49-02, page: 0952.
Contained By:	Masters Abstracts International49-02.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR68610
ISBN:	9780494686102

Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L.
Byczko, Helen Catherine.

Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L. - 90 p.

Source: Masters Abstracts International, Volume: 49-02, page: 0952.

Thesis (M.Sc.)--University of Guelph (Canada), 2010.

A class A4 heat shock transcription factor (Hsf) from Medicago sativa L. was compared to a class A1 and a class A4 Hsf from Arabidopsis thaliana. Transient expression assays with beta-Glucuronidase and Luciferase reporter genes were used in a replicated study comparing mesophyll protoplasts from five species, Nicotiana tobaccum, Arabidopsis, M. sativa, M. truncatula and Lotus japonicus. A significant Hsf protein by protoplast interaction (P=0.0162) was detected. The Arabidopsis class A1 activated significantly (P<0.05) higher levels of reporter compared to the Arabidopsis and Medicago class A4 in Nicotiana protoplasts. In Arabidopsis, and M. truncatula and Lotus protoplasts it was the reverse; the A4 Hsf induced higher expression than the A1. The class A4 Arabidopsis and Medicago had comparable activities in M sativa, M. truncatula and Lotus protoplasts. This study revealed that model plant species have differential response which altered the relative activation of Hsf proteins in the heat shock response.

ISBN: 9780494686102Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L.
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100 1 $a Byczko, Helen Catherine. $3 1677130
245 1 0 $a Gene activation potential of MsHsfA4, a heat shock transcription factor from Medicago sativa L.
300 $a 90 p.
500 $a Source: Masters Abstracts International, Volume: 49-02, page: 0952.
502 $a Thesis (M.Sc.)--University of Guelph (Canada), 2010.
520 $a A class A4 heat shock transcription factor (Hsf) from Medicago sativa L. was compared to a class A1 and a class A4 Hsf from Arabidopsis thaliana. Transient expression assays with beta-Glucuronidase and Luciferase reporter genes were used in a replicated study comparing mesophyll protoplasts from five species, Nicotiana tobaccum, Arabidopsis, M. sativa, M. truncatula and Lotus japonicus. A significant Hsf protein by protoplast interaction (P=0.0162) was detected. The Arabidopsis class A1 activated significantly (P<0.05) higher levels of reporter compared to the Arabidopsis and Medicago class A4 in Nicotiana protoplasts. In Arabidopsis, and M. truncatula and Lotus protoplasts it was the reverse; the A4 Hsf induced higher expression than the A1. The class A4 Arabidopsis and Medicago had comparable activities in M sativa, M. truncatula and Lotus protoplasts. This study revealed that model plant species have differential response which altered the relative activation of Hsf proteins in the heat shock response.
590 $a School code: 0081.
650 4 $a Agriculture, Horticulture. $3 1017832
650 4 $a Agriculture, Plant Culture. $3 1018669
690 $a 0471
690 $a 0479
710 2 $a University of Guelph (Canada). $3 1018650
773 0 $t Masters Abstracts International $g 49-02.
790 $a 0081
791 $a M.Sc.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR68610