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Development of a proteomics-based me...

Holligan, Simone.

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Development of a proteomics-based method for the investigation of bovine pancreatic cell function.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of a proteomics-based method for the investigation of bovine pancreatic cell function./
Author:	Holligan, Simone.
Description:	115 p.
Notes:	Source: Masters Abstracts International, Volume: 48-05, page: 2867.
Contained By:	Masters Abstracts International48-05.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR60395
ISBN:	9780494603956

Development of a proteomics-based method for the investigation of bovine pancreatic cell function.
Holligan, Simone.

Development of a proteomics-based method for the investigation of bovine pancreatic cell function. - 115 p.

Source: Masters Abstracts International, Volume: 48-05, page: 2867.

Thesis (M.Sc.)--University of Guelph (Canada), 2010.

Development of a method to elucidate changes in bovine pancreatic cell function using proteomic tools is described. Two groups of 48 and 45 crossbred steers in years 1 and 2 respectively were fed a high-moisture corn-based diet during finishing and were ranked according to their pancreatic alpha-amylase activity. Steers with high, medium or low alpha-amylase activity were selected, with 3 for each activity range and a total of 9 for each experimental year, for comparison of their proteome profiles. Pancreatic samples from each animal were fractionated using two-dimensional high-performance liquid chromatography and detected using UV spectrophotometry. Software analysis revealed 119 common protein fractions among the 18 animals, and statistical analysis revealed 10 of these fractions differing in abundance between animals with high and low pancreatic alpha-amylase activity. Six protein fractions identified after tandem mass spectrometry analysis and database searches were found to have either protein-binding, DNA-binding or enzymatic capabilities.

ISBN: 9780494603956Subjects--Topical Terms:

1017719
Biology, Molecular.

Development of a proteomics-based method for the investigation of bovine pancreatic cell function.
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020 $a 9780494603956
035 $a (UMI)AAIMR60395
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100 1 $a Holligan, Simone. $3 1677071
245 1 0 $a Development of a proteomics-based method for the investigation of bovine pancreatic cell function.
300 $a 115 p.
500 $a Source: Masters Abstracts International, Volume: 48-05, page: 2867.
502 $a Thesis (M.Sc.)--University of Guelph (Canada), 2010.
520 $a Development of a method to elucidate changes in bovine pancreatic cell function using proteomic tools is described. Two groups of 48 and 45 crossbred steers in years 1 and 2 respectively were fed a high-moisture corn-based diet during finishing and were ranked according to their pancreatic alpha-amylase activity. Steers with high, medium or low alpha-amylase activity were selected, with 3 for each activity range and a total of 9 for each experimental year, for comparison of their proteome profiles. Pancreatic samples from each animal were fractionated using two-dimensional high-performance liquid chromatography and detected using UV spectrophotometry. Software analysis revealed 119 common protein fractions among the 18 animals, and statistical analysis revealed 10 of these fractions differing in abundance between animals with high and low pancreatic alpha-amylase activity. Six protein fractions identified after tandem mass spectrometry analysis and database searches were found to have either protein-binding, DNA-binding or enzymatic capabilities.
590 $a School code: 0081.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
690 $a 0307
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710 2 $a University of Guelph (Canada). $3 1018650
773 0 $t Masters Abstracts International $g 48-05.
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791 $a M.Sc.
792 $a 2010
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