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Identification of a chloroplastic "R...

Arruda, Amanda Sousa.

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Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX)./
Author:	Arruda, Amanda Sousa.
Description:	132 p.
Notes:	Source: Masters Abstracts International, Volume: 48-04, page: 2122.
Contained By:	Masters Abstracts International48-04.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR59285
ISBN:	9780494592854

Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX).
Arruda, Amanda Sousa.

Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX). - 132 p.

Source: Masters Abstracts International, Volume: 48-04, page: 2122.

Thesis (M.Sc.)--University of Toronto (Canada), 2009.

Previous studies have detected the presence of the capsid protein (CP) and CP RNA of PVX in chloroplasts of transgenic plants expressing the PVX CP and 8kD genes from nuclear transgenes. CP RNA was also found in the chloroplast of transgenic plants with mutations that eliminated either the CP or the 8kD protein. To further investigate the potential "RNA Tractor" activity of the PVX RNA, various constructs containing limited regions of the PVX 8kD and CP genes were produced and used to transform tobacco plants. RT-PCR analyses of chloroplastic RNA ascertained the presence of the RNA transcript within chloroplasts when as little as 125bp of the PVX sequence was used to transform plants. From this, it was concluded that the PVX proteins did not contribute to the movement of the viral RNA and that a region within the 125bp PVX sequence is acting as a chloroplastic "RNA Tractor".

ISBN: 9780494592854Subjects--Topical Terms:

1017719
Biology, Molecular.

Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX).
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100 1 $a Arruda, Amanda Sousa. $3 1677065
245 1 0 $a Identification of a chloroplastic "RNA tractor" within the genome of Potato virus X (PVX).
300 $a 132 p.
500 $a Source: Masters Abstracts International, Volume: 48-04, page: 2122.
502 $a Thesis (M.Sc.)--University of Toronto (Canada), 2009.
520 $a Previous studies have detected the presence of the capsid protein (CP) and CP RNA of PVX in chloroplasts of transgenic plants expressing the PVX CP and 8kD genes from nuclear transgenes. CP RNA was also found in the chloroplast of transgenic plants with mutations that eliminated either the CP or the 8kD protein. To further investigate the potential "RNA Tractor" activity of the PVX RNA, various constructs containing limited regions of the PVX 8kD and CP genes were produced and used to transform tobacco plants. RT-PCR analyses of chloroplastic RNA ascertained the presence of the RNA transcript within chloroplasts when as little as 125bp of the PVX sequence was used to transform plants. From this, it was concluded that the PVX proteins did not contribute to the movement of the viral RNA and that a region within the 125bp PVX sequence is acting as a chloroplastic "RNA Tractor".
590 $a School code: 0779.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Agriculture, Plant Culture. $3 1018669
690 $a 0307
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710 2 $a University of Toronto (Canada). $3 1017674
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790 $a 0779
791 $a M.Sc.
792 $a 2009
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