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Assessing new methods of integrated ...

Sisson, Adam James.

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Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa./
Author:	Sisson, Adam James.
Description:	105 p.
Notes:	Source: Masters Abstracts International, Volume: 47-04, page: 2065.
Contained By:	Masters Abstracts International47-04.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1462170
ISBN:	9781109057003

Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa.
Sisson, Adam James.

Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa. - 105 p.

Source: Masters Abstracts International, Volume: 47-04, page: 2065.

Thesis (M.S.)--Iowa State University, 2009.

Sooty blotch and flyspeck (SBFS) is a complex of >60 fungal species that blemish the surface of apple fruit in humid regions worldwide. Blemishes become visible in mid-to late summer, reducing the value of fresh fruit. To test the hypothesis that SBFS species appear on apples at characteristic times during the growing season, 22-37 apples were monitored weekly for appearance of SBFS colonies at each of three Iowa orchards in 2006 and seven orchards in 2007. Colonies were marked with colored pens to denote the date of appearance. After harvest and storage of apples at 4°C for 3 months, SBFS colonies on each fruit were counted and classified by morphology, and a representative subset of colonies with subtending peel was removed and pressed. Fungal DNA, extracted from colonies scraped from the surface of the peel, was amplified with primer pair ITS1-F/Myc1-R. Polymerase chain reaction products were digested with HaeIII, and fragment patterns were observed with gel electrophoresis and compared to a library of previously identified SBFS species. Colonies that could not be identified using band patterns were sequenced and compared to other species using BLAST. Sterile mycelia spp. RS1 and RS2 were the first to appear in all but one of the Iowa orchards surveyed where SBFS signs were observed. Dissoconium aciculare consistently appeared on fruit during the week prior to harvest, and additional colonies of this species appeared during storage. The species that were most prevalent in Iowa orchards were also the most abundant. Knowledge of species prevalence and chronology of appearance on apple fruit could lead to improved SBFS management strategies.

ISBN: 9781109057003Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa.
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100 1 $a Sisson, Adam James. $3 1676920
245 1 0 $a Assessing new methods of integrated pest management for apple orchards in the midwest and phenology of sooty blotch and flyspeck fungi on apples in Iowa.
300 $a 105 p.
500 $a Source: Masters Abstracts International, Volume: 47-04, page: 2065.
500 $a Advisers: Mark L. Gleason; Matthew Liebman.
502 $a Thesis (M.S.)--Iowa State University, 2009.
520 $a Sooty blotch and flyspeck (SBFS) is a complex of >60 fungal species that blemish the surface of apple fruit in humid regions worldwide. Blemishes become visible in mid-to late summer, reducing the value of fresh fruit. To test the hypothesis that SBFS species appear on apples at characteristic times during the growing season, 22-37 apples were monitored weekly for appearance of SBFS colonies at each of three Iowa orchards in 2006 and seven orchards in 2007. Colonies were marked with colored pens to denote the date of appearance. After harvest and storage of apples at 4°C for 3 months, SBFS colonies on each fruit were counted and classified by morphology, and a representative subset of colonies with subtending peel was removed and pressed. Fungal DNA, extracted from colonies scraped from the surface of the peel, was amplified with primer pair ITS1-F/Myc1-R. Polymerase chain reaction products were digested with HaeIII, and fragment patterns were observed with gel electrophoresis and compared to a library of previously identified SBFS species. Colonies that could not be identified using band patterns were sequenced and compared to other species using BLAST. Sterile mycelia spp. RS1 and RS2 were the first to appear in all but one of the Iowa orchards surveyed where SBFS signs were observed. Dissoconium aciculare consistently appeared on fruit during the week prior to harvest, and additional colonies of this species appeared during storage. The species that were most prevalent in Iowa orchards were also the most abundant. Knowledge of species prevalence and chronology of appearance on apple fruit could lead to improved SBFS management strategies.
590 $a School code: 0097.
650 4 $a Agriculture, Horticulture. $3 1017832
650 4 $a Agriculture, Plant Pathology. $3 1028950
650 4 $a Engineering, Agricultural. $3 1019504
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710 2 $a Iowa State University. $b Plant Pathology. $3 1272632
773 0 $t Masters Abstracts International $g 47-04.
790 1 0 $a Gleason, Mark L., $e advisor
790 1 0 $a Liebman, Matthew, $e advisor
790 1 0 $a Domoto, Paul A. $e committee member
790 1 0 $a Lewis, Donald R. $e committee member
790 1 0 $a Duffy, Michael D. $e committee member
790 $a 0097
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1462170