東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Small angle scatting studies of self...

Singh, Divya.

Linked to FindBook

Google Book

Amazon

博客來

Small angle scatting studies of self assembly in lipid mixtures.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Small angle scatting studies of self assembly in lipid mixtures./
Author:	Singh, Divya.
Description:	275 p.
Notes:	Source: Dissertation Abstracts International, Volume: 69-12, Section: B, page: 7355.
Contained By:	Dissertation Abstracts International69-12B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3340003
ISBN:	9780549939870

Small angle scatting studies of self assembly in lipid mixtures.
Singh, Divya.

Small angle scatting studies of self assembly in lipid mixtures. - 275 p.

Source: Dissertation Abstracts International, Volume: 69-12, Section: B, page: 7355.

Thesis (Ph.D.)--The Johns Hopkins University, 2009.

Systems consisting of mixtures of a long and a short-tail lipid, commonly referred to as 'biclles', have recently shown promise in membrane protein crystallization and have been used for some time as an alignable media for use in NMR based protein structure determination. However, the phase behavior of these lipid mixtures remains poorly understood. This dissertation reports small angle scattering studies to gain an understanding of the structure and composition, as well as the mechanism of structural transformation by systematically tailoring those mixtures. A detailed understanding of the phase behavior is essential to adapting them for specific applications.

ISBN: 9780549939870Subjects--Topical Terms:

1019105
Biophysics, General.

Small angle scatting studies of self assembly in lipid mixtures.
LDR:03382nam 2200289 4500 001 1397235
005 20110719084831.5
008 130515s2009 ||||||||||||||||| ||eng d
020 $a 9780549939870
035 $a (UMI)AAI3340003
035 $a AAI3340003
040 $a UMI $c UMI
100 1 $a Singh, Divya. $3 1676052
245 1 0 $a Small angle scatting studies of self assembly in lipid mixtures.
300 $a 275 p.
500 $a Source: Dissertation Abstracts International, Volume: 69-12, Section: B, page: 7355.
500 $a Advisers: Paul Butler; Lionel Porcar.
502 $a Thesis (Ph.D.)--The Johns Hopkins University, 2009.
520 $a Systems consisting of mixtures of a long and a short-tail lipid, commonly referred to as 'biclles', have recently shown promise in membrane protein crystallization and have been used for some time as an alignable media for use in NMR based protein structure determination. However, the phase behavior of these lipid mixtures remains poorly understood. This dissertation reports small angle scattering studies to gain an understanding of the structure and composition, as well as the mechanism of structural transformation by systematically tailoring those mixtures. A detailed understanding of the phase behavior is essential to adapting them for specific applications.
520 $a In DMPC/DHPC (or C14/C6) mixtures at temperatures &sim;10-15°C below the melting transition temperature Tm of the long tail lipid C14, small angle neutron scattering (SANS) experiments with mixtures of hydrogenated and deuterated lipids give direct evidence of the segregated discoidal morphology, while use of standard mixed surfactant theories account to first order for the size of the discs. The deviation of their dilution induced growth from the fully segregated models, an evidence of net mixing of the long lipid into the short lipid rim, is confirmed by contrast enhanced SANS. Previously ignored and unaccounted for, such a mixing behavior is essential for the energetics of a self-assembled mixture of two lipids with vastly different spontaneous curvatures. When the low temperature study is extended to other phospholipid-detergent mixtures, universality of the discoidal phase is observed, highlighting the role of the rigid, gel state long lipid and the edge tension reducing properties of the short lipids in the self-assembly. As a step further, when the size of the smaller lipid species is increased to consist of eight carbon tails, (C14/C8 mixture), elongated tablet like aggregates with fiat, non circular cross-section are the predominant structures, as shown by complimentary SANS and SAXS (small angle X-ray scattering) techniques. Similar structures are also observed in C14/C6 mixtures at temperatures near and above the Tm of C14 lipids. Chain-melting at higher temperatures in C14/C6 mixtures and mixing of the two lipids due to decreasing chain length difference (or hydrophobic mismatch) in C14/C8 are found to be equivalent effects, affecting the interfacial and bulk properties of the aggregates, and leading to preferential growth in one-dimension.
590 $a School code: 0098.
650 4 $a Biophysics, General. $3 1019105
690 $a 0786
710 2 $a The Johns Hopkins University. $3 1017431
773 0 $t Dissertation Abstracts International $g 69-12B.
790 1 0 $a Butler, Paul, $e advisor
790 1 0 $a Porcar, Lionel, $e advisor
790 $a 0098
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3340003