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In Vitro Evaluations of Macrophage-T...

Attarwala, Husain.

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In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations./
Author:	Attarwala, Husain.
Description:	57 p.
Notes:	Source: Masters Abstracts International, Volume: 49-03, page: .
Contained By:	Masters Abstracts International49-03.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1483819
ISBN:	9781124390178

In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations.
Attarwala, Husain.

In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations. - 57 p.

Source: Masters Abstracts International, Volume: 49-03, page: .

Thesis (M.S.)--Northeastern University, 2010.

Purpose: Multi-compartmental delivery systems can effectively deliver therapeutic payloads by overcoming different extra- and intra-cellular barriers. The main objective of this study was to develop DNA-encapsulated solid nanoparticles-in-emulsion formulation to enhance gene delivery and transfection in macrophages.

ISBN: 9781124390178Subjects--Topical Terms:

1017730
Biology, Genetics.

In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations.
LDR:03172nam 2200349 4500 001 1396056
005 20110527105459.5
008 130515s2010 ||||||||||||||||| ||eng d
020 $a 9781124390178
035 $a (UMI)AAI1483819
035 $a AAI1483819
040 $a UMI $c UMI
100 1 $a Attarwala, Husain. $3 1674813
245 1 0 $a In Vitro Evaluations of Macrophage-Targeted Anti-Inflammatory Gene Delivery and Transfection using Nanoparticles-in-Emulsion Formulations.
300 $a 57 p.
500 $a Source: Masters Abstracts International, Volume: 49-03, page: .
500 $a Adviser: Mansoor M. Amiji.
502 $a Thesis (M.S.)--Northeastern University, 2010.
520 $a Purpose: Multi-compartmental delivery systems can effectively deliver therapeutic payloads by overcoming different extra- and intra-cellular barriers. The main objective of this study was to develop DNA-encapsulated solid nanoparticles-in-emulsion formulation to enhance gene delivery and transfection in macrophages.
520 $a Methods: Type B gelatin based nanoparticles were prepared by ethanol water solvent displacement method. Safflower oil-containing water in oil in water multiple emulsion was prepared by a two step emulsification technique with the help of Silverson homogenizer L4RTRTM. EGFP-N1 plasmid DNA that expresses enhanced green fluorescent protein (GFP) was encapsulated in solid type B gelatin nanoparticles (GNP) which were further encapsulated in the innermost aqueous phase of safflower oil containing water in oil in water (W/O/W) multiple emulsion (ME). Fluorescence microscopy and GFP-specific ELISA were performed for qualitative and quantitative determination of plasmid EGFP-N1 gene transfection in murine adherent alveolar macrophages J774A.1.
520 $a Results: Particle size of the NiE formulations was found to be less than or equal to 5 um in diameter. Results obtained from gene transfection evaluations showed that plasmid EGFP-N1 or mIL-10 loaded NiE formulations offered higher and sustained GFP expression compared GFP expression observed with plasmid EGFP-N1 or mLI-10 loaded ME and GNP formulations or plasmid DNA complexed with LipofectinRTM, a cationic lipid based standard gene transfection reagent in J774A.1 murine adherent alveolar macrophage cell lines.
520 $a Conclusion: The results of this study show that DNA-containing solid gelatin nanoparticles can be encapsulated in the innermost aqueous phase of the W/O/W multiple emulsions to form NiE formulations. EGFP-N1 or mIL-10 plasmid-loaded NiE formulations were capable producing sustained gene transfection in J774A.1 murine adherent alveolar macrophage cell lines. In addition mIL10 transfection using NiE formulation showed highest amount of TNF&agr; and IL-1beta suppression upon LPS stimulation.
590 $a School code: 0160.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Health Sciences, Pharmacy. $3 1017737
650 4 $a Health Sciences, Immunology. $3 1017716
690 $a 0369
690 $a 0572
690 $a 0982
710 2 $a Northeastern University. $b Pharmaceutical Sciences. $3 1674814
773 0 $t Masters Abstracts International $g 49-03.
790 1 0 $a Amiji, Mansoor M., $e advisor
790 1 0 $a Gatley, John S. $e committee member
790 1 0 $a Carrier, Rebecca L. $e committee member
790 $a 0160
791 $a M.S.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1483819