東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Sorting of amphiphile membrane compo...

Tian, Aiwei.

Linked to FindBook

Google Book

Amazon

博客來

Sorting of amphiphile membrane components in curvature and composition gradients.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Sorting of amphiphile membrane components in curvature and composition gradients./
Author:	Tian, Aiwei.
Description:	226 p.
Notes:	Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .
Contained By:	Dissertation Abstracts International71-12B.
Subject:	Chemistry, Polymer. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3431179
ISBN:	9781124316765

Sorting of amphiphile membrane components in curvature and composition gradients.
Tian, Aiwei.

Sorting of amphiphile membrane components in curvature and composition gradients. - 226 p.

Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .

Thesis (Ph.D.)--University of Pennsylvania, 2010.

Phase and shape heterogeneities in biomembranes are of functional importance. However, it is difficult to elucidate the roles membrane heterogeneities play in maintaining cellular function due to the complexity of biomembranes. Therefore, investigations of phase behavior and composition/curvature coupling in lipid and polymer model membranes offer some advantages.

ISBN: 9781124316765Subjects--Topical Terms:

1018428
Chemistry, Polymer.

Sorting of amphiphile membrane components in curvature and composition gradients.
LDR:02928nam 2200313 4500 001 1392688
005 20110218114648.5
008 130515s2010 ||||||||||||||||| ||eng d
020 $a 9781124316765
035 $a (UMI)AAI3431179
035 $a AAI3431179
040 $a UMI $c UMI
100 1 $a Tian, Aiwei. $3 1671153
245 1 0 $a Sorting of amphiphile membrane components in curvature and composition gradients.
300 $a 226 p.
500 $a Source: Dissertation Abstracts International, Volume: 71-12, Section: B, page: .
500 $a Adviser: Tobias Baungart.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2010.
520 $a Phase and shape heterogeneities in biomembranes are of functional importance. However, it is difficult to elucidate the roles membrane heterogeneities play in maintaining cellular function due to the complexity of biomembranes. Therefore, investigations of phase behavior and composition/curvature coupling in lipid and polymer model membranes offer some advantages.
520 $a In this thesis, phase properties in lipid and polymer giant vesicles were studied. Line tension at the fluid/fluid phase boundary of giant lipid unilamellar vesicles was determined directly by micropipette aspiration, and found to be composition-dependent. Dynamics of calcium-induced domains within polyanionic vesicles subject to chemical stimuli were investigated, which revealed the strength of molecular interaction and suggested applications in triggered delivery.
520 $a In addition, curvature sorting of lipids and proteins was examined. Lipid membrane tethers were pulled from giant unilamellar vesicles using two micropipettes and a bead. Tether radius can be controlled and measured in this system. By examining fluorescence intensity of labeled molecules as a function of curvature, we found that DiI dyes (lipid analogues with spontaneous curvatures) had no curvature preference down to radii of 10 nm. Theoretical calculation predicted that the distribution of small lipids was dominated by entropy instead of bending energy. However protein Cholera toxin subunit B was efficiently sorted away from the high positive curvature due to its negative spontaneous curvature. Bending stiffness was determined to decrease as curvature increased in homogeneous membranes with ternary lipid mixtures near a critical consulate point, revealing the strong preferential intermolecular interactions of such mixtures. In addition, diffusion controlled domain growth was observed in tethers pulled from phase-separated vesicles, which provides a new dynamic sorting principle for lipids and proteins in curvature gradients.
590 $a School code: 0175.
650 4 $a Chemistry, Polymer. $3 1018428
650 4 $a Engineering, Chemical. $3 1018531
650 4 $a Engineering, Materials Science. $3 1017759
690 $a 0495
690 $a 0542
690 $a 0794
710 2 $a University of Pennsylvania. $3 1017401
773 0 $t Dissertation Abstracts International $g 71-12B.
790 1 0 $a Baungart, Tobias, $e advisor
790 $a 0175
791 $a Ph.D.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3431179