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Comparison of RNA quality from stres...

Salazar, Mary Alice.

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Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli./
Author:	Salazar, Mary Alice.
Description:	22 p.
Notes:	Source: Masters Abstracts International, Volume: 48-05, page: .
Contained By:	Masters Abstracts International48-05.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1475568
ISBN:	9781109747799

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.
Salazar, Mary Alice.

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli. - 22 p.

Source: Masters Abstracts International, Volume: 48-05, page: .

Thesis (M.S.)--Clemson University, 2010.

High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant E. coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of RNA purification-related degradation but could be used as a tool to characterize and detect stressful culture conditions that target ribosomal RNA. Also, these results suggest the need for caution when assessing RNA quality based on ribosomal RNA abundance in stressed cells.

ISBN: 9781109747799Subjects--Topical Terms:

1017719
Biology, Molecular.

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.
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020 $a 9781109747799
035 $a (UMI)AAI1475568
035 $a AAI1475568
040 $a UMI $c UMI
100 1 $a Salazar, Mary Alice. $3 1669143
245 1 0 $a Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.
300 $a 22 p.
500 $a Source: Masters Abstracts International, Volume: 48-05, page: .
500 $a Adviser: Sarah W. Harcum.
502 $a Thesis (M.S.)--Clemson University, 2010.
520 $a High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant E. coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of RNA purification-related degradation but could be used as a tool to characterize and detect stressful culture conditions that target ribosomal RNA. Also, these results suggest the need for caution when assessing RNA quality based on ribosomal RNA abundance in stressed cells.
590 $a School code: 0050.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0307
690 $a 0486
710 2 $a Clemson University. $b Chemistry. $3 1030764
773 0 $t Masters Abstracts International $g 48-05.
790 1 0 $a Harcum, Sarah W., $e advisor
790 1 0 $a Christensen, Kenneth $e committee member
790 1 0 $a Chumanov, George $e committee member
790 $a 0050
791 $a M.S.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1475568