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Comparison of RNA quality from stres...

Salazar, Mary Alice.

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Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli./
作者:	Salazar, Mary Alice.
面頁冊數:	22 p.
附註:	Source: Masters Abstracts International, Volume: 48-05, page: .
Contained By:	Masters Abstracts International48-05.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1475568
ISBN:	9781109747799

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.
Salazar, Mary Alice.

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli. - 22 p.

Source: Masters Abstracts International, Volume: 48-05, page: .

Thesis (M.S.)--Clemson University, 2010.

High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant E. coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of RNA purification-related degradation but could be used as a tool to characterize and detect stressful culture conditions that target ribosomal RNA. Also, these results suggest the need for caution when assessing RNA quality based on ribosomal RNA abundance in stressed cells.

ISBN: 9781109747799Subjects--Topical Terms:

1017719
Biology, Molecular.

Comparison of RNA quality from stressed and unstressed recombinant Escherichia coli.
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