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Characterization of a bacterial arti...

McDowell, Erin.

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Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988./
Author:	McDowell, Erin.
Description:	105 p.
Notes:	Source: Masters Abstracts International, Volume: 48-01, page: 0317.
Contained By:	Masters Abstracts International48-01.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1469505
ISBN:	9781109386011

Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988.
McDowell, Erin.

Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988. - 105 p.

Source: Masters Abstracts International, Volume: 48-01, page: 0317.

Thesis (M.S.)--University of Delaware, 2009.

Maek's Disease (MD) is a transmissible herpesvirus infection that is characterized by paralysis, T-cell lymphoma formation and immunosuppresion in chickens. The causative agent, Marek's Disease virus (MDV), is a cell-associated alphaherpesvirus, sub-species Mardivirus. MD is controlled in commercial poultry production through vaccination, but field strains of MDV continue to evolve in virulence. The most efficacious vaccine commercially available is CVI988 (Rispens) that was developed as a vaccine after attenuation in cell culture at passage 33 (p33). As CVI988 vaccines from different suppliers vary in efficacy, we sought to construct a bacterial artificial chromosome (BAC)-bases infectious clone of low passage (<p25). DNA from p19 CVI988 was obtained from the Central Veterinary Institute, Lelystad via a collaborator in France. This DNA was co-transfected into CEF along with BAC transfer vector (pDS-HA-1), passaged in selection, and subsequent BAC-containing viruses were electroporated into E.coli to give rise to CVI988-BAC clones. One particular clone, CVI988-BAC, 699-2 (isolated from chicken #699) had the BAC sequences subsequently deleted through Cre-mediated recombination to yield CVI988, 699-2Delta. This BAC-deleted virus was subsequently tested in three large-scale efficacy trials and was found to provide protection against a vv+MDV challenge with efficacy superior to the commercial CVI988 vaccines tested. Upon serial back-passage in specific pathogen free (SPF) leghorn chickens however, CVI988, 699-2Delta induced microscopic tumors at back passages 3 and 5, and when administered at a 10X dose indicating a reversion to virulence. Our data demonstrate that very minute differences exist between a highly-efficacious vaccine and one that has pathogenic potential.

ISBN: 9781109386011Subjects--Topical Terms:

1017719
Biology, Molecular.

Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988.
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245 1 0 $a Characterization of a bacterial artificial chromosome (BAC)-based infectious clone of a low passage Marek's disease virus (MDV) vaccine strain, CVI988.
300 $a 105 p.
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520 $a Maek's Disease (MD) is a transmissible herpesvirus infection that is characterized by paralysis, T-cell lymphoma formation and immunosuppresion in chickens. The causative agent, Marek's Disease virus (MDV), is a cell-associated alphaherpesvirus, sub-species Mardivirus. MD is controlled in commercial poultry production through vaccination, but field strains of MDV continue to evolve in virulence. The most efficacious vaccine commercially available is CVI988 (Rispens) that was developed as a vaccine after attenuation in cell culture at passage 33 (p33). As CVI988 vaccines from different suppliers vary in efficacy, we sought to construct a bacterial artificial chromosome (BAC)-bases infectious clone of low passage (<p25). DNA from p19 CVI988 was obtained from the Central Veterinary Institute, Lelystad via a collaborator in France. This DNA was co-transfected into CEF along with BAC transfer vector (pDS-HA-1), passaged in selection, and subsequent BAC-containing viruses were electroporated into E.coli to give rise to CVI988-BAC clones. One particular clone, CVI988-BAC, 699-2 (isolated from chicken #699) had the BAC sequences subsequently deleted through Cre-mediated recombination to yield CVI988, 699-2Delta. This BAC-deleted virus was subsequently tested in three large-scale efficacy trials and was found to provide protection against a vv+MDV challenge with efficacy superior to the commercial CVI988 vaccines tested. Upon serial back-passage in specific pathogen free (SPF) leghorn chickens however, CVI988, 699-2Delta induced microscopic tumors at back passages 3 and 5, and when administered at a 10X dose indicating a reversion to virulence. Our data demonstrate that very minute differences exist between a highly-efficacious vaccine and one that has pathogenic potential.
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