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Fiber-optic ammonia sensors designed...

Kar, Satyajit.

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Fiber-optic ammonia sensors designed for synaptic ammonia measurements.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Fiber-optic ammonia sensors designed for synaptic ammonia measurements./
Author:	Kar, Satyajit.
Description:	210 p.
Notes:	Source: Dissertation Abstracts International, Volume: 53-11, Section: B, page: 5681.
Contained By:	Dissertation Abstracts International53-11B.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9308070

Fiber-optic ammonia sensors designed for synaptic ammonia measurements.
Kar, Satyajit.

Fiber-optic ammonia sensors designed for synaptic ammonia measurements. - 210 p.

Source: Dissertation Abstracts International, Volume: 53-11, Section: B, page: 5681.

Thesis (Ph.D.)--The University of Iowa, 1992.

The goal of this research project is to develop a fiber-optic ammonia sensor (FOAS) which can be used as the internal sensing unit of a biosensor for measuring synaptic glutamate during neurochemical experiments. The glutamate biosensor is constructed by immobilizing glutamate oxidase at the sensing tip of a FOAS. The glutamate concentration of interest ranges from 1 to 10 $\muSubjects--Topical Terms:

586156
Chemistry, Analytical.

Fiber-optic ammonia sensors designed for synaptic ammonia measurements.
LDR:03038nam 2200289 a 45 001 971761
005 20110927
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035 $a (UnM)AAI9308070
035 $a AAI9308070
040 $a UnM $c UnM
100 1 $a Kar, Satyajit. $3 1295791
245 1 0 $a Fiber-optic ammonia sensors designed for synaptic ammonia measurements.
300 $a 210 p.
500 $a Source: Dissertation Abstracts International, Volume: 53-11, Section: B, page: 5681.
500 $a Supervisor: Mark A. Arnold.
502 $a Thesis (Ph.D.)--The University of Iowa, 1992.
520 $a The goal of this research project is to develop a fiber-optic ammonia sensor (FOAS) which can be used as the internal sensing unit of a biosensor for measuring synaptic glutamate during neurochemical experiments. The glutamate biosensor is constructed by immobilizing glutamate oxidase at the sensing tip of a FOAS. The glutamate concentration of interest ranges from 1 to 10 $\mu $m which corresponds to an ammonia concentration range from 34-340 nM ammonia under physiological conditions.
520 $a Three types of FOAS's have been designed. In the first two, a small volume of internal solution is trapped in front of a fiber-optic probe (FOP) with either an air-gap or a gas permeable membrane. In the third case, the internal solution is trapped inside a gas-permeable tubing with two FOP's. The internal solution is composed of either a pH sensitive fluorophore or chromophore dissolved in a solution of ammonium chloride. Ammonia from the sample diffuses across the membrane and reacts with the indicator. The non-protonated form of the indicator is detected by either a fluorescence or absorbance measurement through the FOP.
520 $a Parameters evaluated in terms of effect on the steady-state and dynamic responses of the membrane based sensor include the indicator dye, concentration of the indicator and total ammonia nitrogen in the internal solution, volume of the internal solution, structure of the gas-permeable membrane, and temperature. Similar studies were performed with air-gap and tubular shaped sensors.
520 $a The membrane based FOAS possesses a limit of detection of 7 nM with response times ranging from 2 to 5 minutes. Glutamate oxidase is immobilized at the tip of this FOAS to provide a glutamate biosensor with a detection limit of 0.1 $\mu $m when operated at pH 7.8. In addition, this ammonia sensor is used to measure extracellular ammonia levels in perfused retinal and eye-cup preparations. These measurements indicate a calcium-dependent release of ammonia during potassium-evoked depolarization of these cells. The miniature air-gap FOAS's possess similar response properties. The tubular shaped FOAS is capable of continuous on line monitoring of ammonia.
590 $a School code: 0096.
650 4 $a Chemistry, Analytical. $3 586156
690 $a 0486
710 2 0 $a The University of Iowa. $3 1017439
773 0 $t Dissertation Abstracts International $g 53-11B.
790 $a 0096
790 1 0 $a Arnold, Mark A., $e advisor
791 $a Ph.D.
792 $a 1992
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9308070