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Identification and stability of anth...

Dee, Stacy.

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Identification and stability of anthocyanins in Plantago lanceolata.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Identification and stability of anthocyanins in Plantago lanceolata./
作者:	Dee, Stacy.
面頁冊數:	87 p.
附註:	Adviser: Nadja B. Cech.
Contained By:	Masters Abstracts International46-01.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1445535
ISBN:	9780549136316

Identification and stability of anthocyanins in Plantago lanceolata.
Dee, Stacy.

Identification and stability of anthocyanins in Plantago lanceolata. - 87 p.

Adviser: Nadja B. Cech.

Thesis (M.S.)--The University of North Carolina at Greensboro, 2007.

Several laboratories have analyzed the content and stability of anthocyanins from foods and flowering plants ranging from potatoes to petunias. Previous research in the Cech and Lacey laboratories tentatively identified anthocyanins in Plantago lanceolata using reversed phase high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS). The goal of this research was to gain more information about the identification of these anthocyanins using hydrolysis and nuclear magnetic resonance (NMR) and to test the stability of these anthocyanins when stored for various periods of time at three different temperatures (room temperature, -20, -80 °C). Repeatability studies were also conducted to validate the analytical method.

ISBN: 9780549136316Subjects--Topical Terms:

586156
Chemistry, Analytical.

Identification and stability of anthocyanins in Plantago lanceolata.
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520 $a Several laboratories have analyzed the content and stability of anthocyanins from foods and flowering plants ranging from potatoes to petunias. Previous research in the Cech and Lacey laboratories tentatively identified anthocyanins in Plantago lanceolata using reversed phase high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS). The goal of this research was to gain more information about the identification of these anthocyanins using hydrolysis and nuclear magnetic resonance (NMR) and to test the stability of these anthocyanins when stored for various periods of time at three different temperatures (room temperature, -20, -80 °C). Repeatability studies were also conducted to validate the analytical method.
520 $a Hydrolysis experiments and analysis by HPLC-ESI-MS confirmed the presence of cyanidin and peonidin glycosides in the original P. lanceolata extract. The most abundant anthocyanin was isolated from P. lanceolata, but attempts to collect an NMR spectrum of this sample were not successful due to the small yield and apparent low purity of the sample isolated. The method variability from injection to injection on the same day was determined to be 10--20%, variability from day to day on the same extract was 30% and the variability from extraction to extraction on different days was 30--60% for cyanidin glycosides a and c, delphinidin glycoside b, peonidin glycoside a, and petunidin glycoside b. Stability studies revealed that there was no degradation that exceeded the variability of the method from day to day in both the -20 and -80°C temperatures for any of the anthocyanins. There was however, significant degradation in the room temperature condition for the cyanidin glycosides a and c.
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