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An analytical method to quantify 1,3...

Foto, Mark S.

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An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay./
作者:	Foto, Mark S.
面頁冊數:	88 p.
附註:	Adviser: J. David Miller.
Contained By:	Masters Abstracts International42-01.
標題:	Chemistry, Analytical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ79810
ISBN:	9780612798106

An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay.
Foto, Mark S.

An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay. - 88 p.

Adviser: J. David Miller.

Thesis (M.Sc.)--Carleton University (Canada), 2003.

The use of 1,3-beta-D-glucan for the determination of fungi in indoor air and dust in research studies has been increasing steadily over the last decade. A very sensitive glucan-specific assay exists, however its cost makes the determination of 1,3-beta-D-glucan in large-scale studies financially difficult. Hence, 1,3-beta-D-glucan was quantitatively measured by developing chemical procedures to adapt the relatively inexpensive Limulus amebocyte lysate assay (commonly used for LPS endotoxin) to 1,3-beta-D-glucan.

ISBN: 9780612798106Subjects--Topical Terms:

586156
Chemistry, Analytical.

An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay.
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245 1 3 $a An analytical method to quantify 1,3-beta-(D)-glucan using the LAL assay.
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500 $a Source: Masters Abstracts International, Volume: 42-01, page: 0233.
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520 $a The use of 1,3-beta-D-glucan for the determination of fungi in indoor air and dust in research studies has been increasing steadily over the last decade. A very sensitive glucan-specific assay exists, however its cost makes the determination of 1,3-beta-D-glucan in large-scale studies financially difficult. Hence, 1,3-beta-D-glucan was quantitatively measured by developing chemical procedures to adapt the relatively inexpensive Limulus amebocyte lysate assay (commonly used for LPS endotoxin) to 1,3-beta-D-glucan.
520 $a 0.5 N NaOH showed best results as an extracting solvent for samples containing both endotoxin and 1,3-beta-D-glucan, with no significant difference in calculated LAL concentration of a known amount of 1,3-beta- D-glucan (curdlan) when present with concentrations of endotoxin below 5ng/mL. The modified LAL method was also useful in analyzing other materials. The glucan content of ragweed pollen (Ambrosia atemisiifolia) was found to be comparable to values obtained by others with the glucan-specific test (Rylander et al., 1999a).
520 $a Size-Exclusion Chromatography was explored with curdlan standards to confirm the size of insoluble 1,3-beta-D-glucans. The correlation between the LAL assay and SEC method (based on concentration and area, respectively) was 0.517 (p < 0.000) over a full year of field samples. However, this correlation increased to 0.822 (p < 0.006) at 180--190 kDa when SEC samples were grouped in 10 kDa ranges. Strong correlations were also seen between 1,3-beta-D-glucan/ergosterol, and 1,3-beta-D-glucan/mold area (0.873, p < 0.000 and 0.763, p < 0.000, respectively). A moderate correlation was observed between 1,3-beta-D-glucan/spore counts (0.501, p < 0.011).
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