東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁 到查詢結果 [ null ]

切換: 標籤 | MARC模式 | ISBD

TGF-beta is a common mediator for po...

Wu, Dona Tsihwa.

FindBook

Google Book

Amazon

博客來

TGF-beta is a common mediator for podocyte differentiation and apoptosis.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	TGF-beta is a common mediator for podocyte differentiation and apoptosis./
作者:	Wu, Dona Tsihwa.
面頁冊數:	198 p.
附註:	Adviser: Erwin P. Bottinger.
Contained By:	Dissertation Abstracts International67-03B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3209656
ISBN:	9780542579400

TGF-beta is a common mediator for podocyte differentiation and apoptosis.
Wu, Dona Tsihwa.

TGF-beta is a common mediator for podocyte differentiation and apoptosis. - 198 p.

Adviser: Erwin P. Bottinger.

Thesis (Ph.D.)--Yeshiva University, 2006.

Podocytes, highly specialized cells in the kidney which regulate plasma filtration, are often injured and lost in glomerular disease. The underlying mechanisms for growth-arrest, differentiation, and depletion of podocytes during renal development and disease remain poorly understood, but may involve a factor that is typically expressed in developing/injured kidneys, transforming growth factor-beta (TGF-beta).

ISBN: 9780542579400Subjects--Topical Terms:

1017686
Biology, Cell.

TGF-beta is a common mediator for podocyte differentiation and apoptosis.
LDR:03539nam 2200313 a 45 001 967343
005 20110915
008 110915s2006 eng d
020 $a 9780542579400
035 $a (UMI)AAI3209656
035 $a AAI3209656
040 $a UMI $c UMI
100 1 $a Wu, Dona Tsihwa. $3 1291220
245 1 0 $a TGF-beta is a common mediator for podocyte differentiation and apoptosis.
300 $a 198 p.
500 $a Adviser: Erwin P. Bottinger.
500 $a Source: Dissertation Abstracts International, Volume: 67-03, Section: B, page: 1242.
502 $a Thesis (Ph.D.)--Yeshiva University, 2006.
520 $a Podocytes, highly specialized cells in the kidney which regulate plasma filtration, are often injured and lost in glomerular disease. The underlying mechanisms for growth-arrest, differentiation, and depletion of podocytes during renal development and disease remain poorly understood, but may involve a factor that is typically expressed in developing/injured kidneys, transforming growth factor-beta (TGF-beta).
520 $a We used a unique system of conditionally-immortalized podocytes which can be stimulated to differentiate to investigate the roles that TGF-beta may play in the regulation of podocyte differentiation and disease. We found that these cells produce Tgf-beta2 at a low level, inducing G0/G1-arrest and differentiation through Smad3-dependent induction of the cyclin dependent kinase inhibitor p15Ink4b (Cdkn2b). However, high concentrations exogenous TGF-beta1 or TGF-beta2 alter these effects by suppressing Cdkn2b, inducing Gadd45beta, and activating proapoptotic p38 MAPK, leading to apoptosis. Thus, we identify distinct signaling profiles activated in a concentration-dependent manner by TGF-beta, suggesting a critical TGF-beta concentration threshold which switches to proapoptotic signaling profiles and apoptosis from podocyte maturation. Thus, TGF-beta may act as a common mediator for podocyte response to injury, and its concentration may ultimately specify the fate of the podocyte.
520 $a We used microarray technology to identify genes that are selectively expressed in differentiated and undifferentiated podocytes and mesangial cells, and to test whether glomerular cell-selective transcriptomes can be used for molecular classification in a mouse models. RNA samples derived from glomerular- or tubular-enriched tissue, primary mesangial cell cultures, and undifferentiated and differentiated podocytes were probed with Affymetrix Genechips(TM). Cell-selective transcripts were derived using current normalization and subtraction schemes. Podocyte-selective transcript sets, when applied to microarray data from the kidneys of a Tgf-beta-transgenic mouse model for progressive glomerulosclerosis, proved sufficient to classify animals according to phenotype. When cell type-selective transcriptomes were combined, classification by phenotype severity was achieved with 100% statistical support. These studies contribute to our understanding of how TGF-beta may regulate podocyte phenotype and provides candidate genes for downstream targets. In this way, we have increased our understanding of the mechanisms which underlie renal pathology and generated tools for future investigations.
590 $a School code: 0266.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0369
690 $a 0379
710 2 0 $a Yeshiva University. $3 1017732
773 0 $t Dissertation Abstracts International $g 67-03B.
790 $a 0266
790 1 0 $a Bottinger, Erwin P., $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3209656