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Mass spectrometric analysis of cytop...

Hays, Faith A.

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Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines./
Author:	Hays, Faith A.
Description:	119 p.
Notes:	Adviser: Catherine Fenselau.
Contained By:	Dissertation Abstracts International67-06B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3222330
ISBN:	9780542731648

Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines.
Hays, Faith A.

Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines. - 119 p.

Adviser: Catherine Fenselau.

Thesis (Ph.D.)--University of Maryland, College Park, 2006.

The average number of ribosomes per cell was determined for the drug susceptible, as well as the drug resistant cell line, and found to be unchanged.

ISBN: 9780542731648Subjects--Topical Terms:

1017686
Biology, Cell.

Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines.
LDR:02523nam 2200325 a 45 001 965359
005 20110906
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020 $a 9780542731648
035 $a (UnM)AAI3222330
035 $a AAI3222330
040 $a UnM $c UnM
100 1 $a Hays, Faith A. $3 1288134
245 1 0 $a Mass spectrometric analysis of cytoplasmic ribosomal proteins in drug resistant and drug susceptible cell lines.
300 $a 119 p.
500 $a Adviser: Catherine Fenselau.
500 $a Source: Dissertation Abstracts International, Volume: 67-06, Section: B, page: 3115.
502 $a Thesis (Ph.D.)--University of Maryland, College Park, 2006.
520 $a The average number of ribosomes per cell was determined for the drug susceptible, as well as the drug resistant cell line, and found to be unchanged.
520 $a This study examines changes in cytoplasmic ribosomes that accompany drug resistance in MCF-7 breast cancer cells. Differences in ribosomal protein composition between drug susceptible and drug resistant cell lines were examined. Ribosomes were isolated from mitoxantrone susceptible and mitoxantrone resistant MCF-7 cells. The acid extracted ribosomal proteins were subjected to optimized 2DGE using a "zoom" strip (pl 7-11) for the first dimension separation. Further optimization of 2DGE included the use of a 15mM DTT wick at the cathode end of the focusing tray, decreasing the protein loading amount and using large format gels for the second dimension. Forty-nine ribosomal proteins were identified in the drug susceptible cell line. Two novel protein isoforms of the proteins RPS3 and one novel isoform of RPS10 were identified in the drug resistant cell line.
520 $a Methods for the extraction and detection of ribosomal proteins from the 2D gel were developed. The method of Mirza et al. was modified and used to extract ribosomal proteins from the gel. The detection of these proteins was optimized by the use of 50% ACN/1.0% TFA to solubilize the MALDI matrix. In addition, the extracted protein solution was mixed 1:1 with 5% Triton X-100. Intact molecular weights were determined for 41 ribosomal proteins using high performance MALDI-TOF mass spectrometry.
590 $a School code: 0117.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Health Sciences, Oncology. $3 1018566
650 4 $a Health Sciences, Pharmacology. $3 1017717
690 $a 0379
690 $a 0419
690 $a 0487
690 $a 0992
710 2 0 $a University of Maryland, College Park. $3 657686
773 0 $t Dissertation Abstracts International $g 67-06B.
790 $a 0117
790 1 0 $a Fenselau, Catherine, $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3222330