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Uncovering factors that alter "free"...

Rangel, Natalie Ann.

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Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR)./
Author:	Rangel, Natalie Ann.
Description:	117 p.
Notes:	Adviser: Chandra Srinivasan.
Contained By:	Masters Abstracts International46-03.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1449339
ISBN:	9780549306849

Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR).
Rangel, Natalie Ann.

Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR). - 117 p.

Adviser: Chandra Srinivasan.

Thesis (M.S.)--California State University, Fullerton, 2008.

Oxidative stress, caused by an over accumulation of reactive oxygen species in the body, is thought to contribute to human aging and the development of age-related diseases. However, free radicals are very difficult to measure directly, so alternative indirect methods must be used for measuring oxidative stress levels. One technique that has been used successfully in Escherichia coli and yeast is based upon the proposed connection between elevated superoxide levels and the release of iron from solvent-exposed [4Fe-4S] clusters. A positive correlation was found in E. coli and yeast between superoxide production within the organism and electron paramagnetic resonance (EPR) detectable "free" Fe (III). Most recently, we have extended the EPR methodology to a higher multicellular system, Caenorhabditis elegans. In this study, we validated the steps previously incorporated into our EPR methodology for C. elegans. We have also shown that this technique can be used to indirectly measure in vivo levels of reactive oxygen species. "Free" iron levels increased when oxidative stress was induced through superoxide production, hydrogen peroxide exposure, heat shock, or altered growth temperatures. Levels of "free" Fe (III) were also shown to be slightly higher in the severely compromised and short-lived C. elegans strain mev-1, a known oxidative stress sensitive mutant. Additionally, supplementation with manganese, a hypothesized antioxidant, was shown to slightly reduce "free" iron levels. The mRNA levels of certain metal homeostasis and antioxidant enzymes were also altered by various treatments.

ISBN: 9780549306849Subjects--Topical Terms:

586156
Chemistry, Analytical.

Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR).
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020 $a 9780549306849
035 $a (UMI)AAI1449339
035 $a AAI1449339
040 $a UMI $c UMI
100 1 $a Rangel, Natalie Ann. $3 1286042
245 1 0 $a Uncovering factors that alter "free" iron levels in Caenorhabditis elegans as measured by electron paramagnetic resonance spectroscopy (EPR).
300 $a 117 p.
500 $a Adviser: Chandra Srinivasan.
500 $a Source: Masters Abstracts International, Volume: 46-03, page: 1523.
502 $a Thesis (M.S.)--California State University, Fullerton, 2008.
520 $a Oxidative stress, caused by an over accumulation of reactive oxygen species in the body, is thought to contribute to human aging and the development of age-related diseases. However, free radicals are very difficult to measure directly, so alternative indirect methods must be used for measuring oxidative stress levels. One technique that has been used successfully in Escherichia coli and yeast is based upon the proposed connection between elevated superoxide levels and the release of iron from solvent-exposed [4Fe-4S] clusters. A positive correlation was found in E. coli and yeast between superoxide production within the organism and electron paramagnetic resonance (EPR) detectable "free" Fe (III). Most recently, we have extended the EPR methodology to a higher multicellular system, Caenorhabditis elegans. In this study, we validated the steps previously incorporated into our EPR methodology for C. elegans. We have also shown that this technique can be used to indirectly measure in vivo levels of reactive oxygen species. "Free" iron levels increased when oxidative stress was induced through superoxide production, hydrogen peroxide exposure, heat shock, or altered growth temperatures. Levels of "free" Fe (III) were also shown to be slightly higher in the severely compromised and short-lived C. elegans strain mev-1, a known oxidative stress sensitive mutant. Additionally, supplementation with manganese, a hypothesized antioxidant, was shown to slightly reduce "free" iron levels. The mRNA levels of certain metal homeostasis and antioxidant enzymes were also altered by various treatments.
590 $a School code: 6060.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
690 $a 0487
710 2 $a California State University, Fullerton. $3 1017845
773 0 $t Masters Abstracts International $g 46-03.
790 $a 6060
790 1 0 $a Srinivasan, Chandra, $e advisor
791 $a M.S.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1449339