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Studies on transplantation of human ...

Ouyang, Edwin Chun.

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Studies on transplantation of human hepatocytes into genetically immunocompetent rodents.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Studies on transplantation of human hepatocytes into genetically immunocompetent rodents./
Author:	Ouyang, Edwin Chun.
Description:	125 p.
Notes:	Adviser: G. Y. Wu.
Contained By:	Dissertation Abstracts International62-03B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3008128
ISBN:	049317026X

Studies on transplantation of human hepatocytes into genetically immunocompetent rodents.
Ouyang, Edwin Chun.

Studies on transplantation of human hepatocytes into genetically immunocompetent rodents. - 125 p.

Adviser: G. Y. Wu.

Thesis (Ph.D.)--The University of Connecticut, 2001.

The objective of this project is to study the feasibility of transplantation of human hepatocytes into immunocompetent rats and test the engraftment, survival and functioning of the transplanted cells. To avoid rejection of human hepatocytes by the rat immune system, tolerance was induced by injection of human hepatocytes into the peritoneal cavities of fetal rats during late gestation. Human hepatocytes were subsequently transplanted into tolerized rat livers via intrasplenic injection within 24 hrs after the birth. Engraftment of donor hepatocytes in the recipient livers was visualized by immunohistochemical staining for human albumin. PCR of genomic DNA and dot blotting results verified that transplanted human hepatocytes survived in rat liver for at least 16 weeks post-transplantation. Human hepatocytes sustained biological functions such as active transcription of human albumin mRNA and secretion of human albumin into the circulation. Transplanted human hepatocytes were found to be susceptible to HBV inoculation. HBV viremia was confirmed by detection of HBV cccDNA and HBsAg in the rat liver and serum. Elevated rat serum ALT levels and changes in liver histology following the HBV inoculation indicated possible hepatic inflammation in the host livers. In conclusion, we established a chimeric human-rodent liver model by prenatal tolerization and post-natal transplantation. This model could be used to study human hepatitis, liver biology, and transplantation immunology.

ISBN: 049317026XSubjects--Topical Terms:

1017686
Biology, Cell.

Studies on transplantation of human hepatocytes into genetically immunocompetent rodents.
LDR:02380nam 2200277 a 45 001 935962
005 20110510
008 110510s2001 eng d
020 $a 049317026X
035 $a (UnM)AAI3008128
035 $a AAI3008128
040 $a UnM $c UnM
100 1 $a Ouyang, Edwin Chun. $3 1259662
245 1 0 $a Studies on transplantation of human hepatocytes into genetically immunocompetent rodents.
300 $a 125 p.
500 $a Adviser: G. Y. Wu.
500 $a Source: Dissertation Abstracts International, Volume: 62-03, Section: B, page: 1180.
502 $a Thesis (Ph.D.)--The University of Connecticut, 2001.
520 $a The objective of this project is to study the feasibility of transplantation of human hepatocytes into immunocompetent rats and test the engraftment, survival and functioning of the transplanted cells. To avoid rejection of human hepatocytes by the rat immune system, tolerance was induced by injection of human hepatocytes into the peritoneal cavities of fetal rats during late gestation. Human hepatocytes were subsequently transplanted into tolerized rat livers via intrasplenic injection within 24 hrs after the birth. Engraftment of donor hepatocytes in the recipient livers was visualized by immunohistochemical staining for human albumin. PCR of genomic DNA and dot blotting results verified that transplanted human hepatocytes survived in rat liver for at least 16 weeks post-transplantation. Human hepatocytes sustained biological functions such as active transcription of human albumin mRNA and secretion of human albumin into the circulation. Transplanted human hepatocytes were found to be susceptible to HBV inoculation. HBV viremia was confirmed by detection of HBV cccDNA and HBsAg in the rat liver and serum. Elevated rat serum ALT levels and changes in liver histology following the HBV inoculation indicated possible hepatic inflammation in the host livers. In conclusion, we established a chimeric human-rodent liver model by prenatal tolerization and post-natal transplantation. This model could be used to study human hepatitis, liver biology, and transplantation immunology.
590 $a School code: 0056.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Health Sciences, Immunology. $3 1017716
690 $a 0379
690 $a 0982
710 2 0 $a The University of Connecticut. $3 1249323
773 0 $t Dissertation Abstracts International $g 62-03B.
790 $a 0056
790 1 0 $a Wu, G. Y., $e advisor
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3008128