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Molecular tagging of male-fertility ...

Gokce, Ali Fuat.

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Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.).

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.)./
作者:	Gokce, Ali Fuat.
面頁冊數:	246 p.
附註:	Source: Dissertation Abstracts International, Volume: 62-11, Section: B, page: 4943.
Contained By:	Dissertation Abstracts International62-11B.
標題:	Agriculture, Plant Culture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3033258
ISBN:	0493460993

Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.).
Gokce, Ali Fuat.

Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.). - 246 p.

Source: Dissertation Abstracts International, Volume: 62-11, Section: B, page: 4943.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2001.

Cytoplasmic-genic male sterility (CMS) is used to produce hybrid-onion seed. For the most widely used source of onion CMS, male sterility is conditioned by the interaction of the male-sterile (S) cytoplasm and the homozygous recessive genotype at a single nuclear male-fertility restoration locus (<italic>Ms </italic>). Maintainer lines possess normal (N) male-fertile cytoplasm and are homozyous recessive at the <italic>Ms</italic> locus. It takes four to eight years to establish if maintainer lines can be extracted from an uncharacterized onion population. Polymorphisms distinguishing N and S cytoplasms of onion have been identified and significantly reduce the time required to establish the cytoplasms of single plants. Segregations of restriction fragment length polymorphisms (RFLPs) revealed RFLPs flanking the <italic>Ms</italic> locus at 0.9 and 8.6 cM. The RFLPs and <italic>Ms</italic> were at or near linkage equilibrium in three open-pollinated onion populations.

ISBN: 0493460993Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.).
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100 1 $a Gokce, Ali Fuat. $3 1256309
245 1 0 $a Molecular tagging of male-fertility restoration locus and its selection in onion (Allium cepa L.).
300 $a 246 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-11, Section: B, page: 4943.
500 $a Supervisor: Michael J. Havey.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2001.
520 $a Cytoplasmic-genic male sterility (CMS) is used to produce hybrid-onion seed. For the most widely used source of onion CMS, male sterility is conditioned by the interaction of the male-sterile (S) cytoplasm and the homozygous recessive genotype at a single nuclear male-fertility restoration locus (<italic>Ms </italic>). Maintainer lines possess normal (N) male-fertile cytoplasm and are homozyous recessive at the <italic>Ms</italic> locus. It takes four to eight years to establish if maintainer lines can be extracted from an uncharacterized onion population. Polymorphisms distinguishing N and S cytoplasms of onion have been identified and significantly reduce the time required to establish the cytoplasms of single plants. Segregations of restriction fragment length polymorphisms (RFLPs) revealed RFLPs flanking the <italic>Ms</italic> locus at 0.9 and 8.6 cM. The RFLPs and <italic>Ms</italic> were at or near linkage equilibrium in three open-pollinated onion populations.
520 $a Using the cDNA sequence (AOB272) revealing the most closely linked RFLP, we designed oligonucleotides and used the polymerase chain reaction to amplify genomic DNAs from seven commercial onion cultivars and the parents (Ailsa Craig 43 and Brigham Yellow Globe 15–23) of our mapping family. We cloned and sequenced these amplified genomic DNAs to identify single nucleotide polymorphisms (SNP). Oligonucleotides were designed flanking the SNPs and were used as allele-specific primers to distinguish genotypes in the AOB272 genomic region. These organellar and nuclear markers can be used to select maintainer genotypes from onion populations or segregating families and should reduce the number of testcrosses required to identify maintaining genotypes.
520 $a The bulb onion is a diploid species with natural outcrossing rates ranging between 70% to 100% in the field. Male sterile plants must receive the male gametes from male-fertile plants in order to set seed. Therefore, the frequency of the <italic>ms</italic> allele will change over generations due to natural selection of male gametes on male-sterile (S-<italic>museums</italic>) plants in onion populations possessing S cytoplasm or mixtures of N and S cytoplasms. I developed a model to predict changes in allied frequencies due to failure of male gamete production from S-<italic>museums</italic> genotypic plants in onion.
590 $a School code: 0262.
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0479
710 2 0 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 62-11B.
790 $a 0262
790 1 0 $a Havey, Michael J., $e advisor
791 $a Ph.D.
792 $a 2001
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