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Tailoring of poly (3-hydroxybutyrate...

Howard University.

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Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications./
Author:	Liu, Hui.
Description:	118 p.
Notes:	Adviser: D. Raghavan.
Contained By:	Dissertation Abstracts International69-11B.
Subject:	Chemistry, Polymer. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3335311
ISBN:	9780549883142

Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications.
Liu, Hui.

Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications. - 118 p.

Adviser: D. Raghavan.

Thesis (Ph.D.)--Howard University, 2008.

Considerable scientific and technological progress has been made in formulating biomaterials based on natural and synthetic polymers for ultimate use in bone tissue scaffolding. One class of polymers that has drawn attention in recent years for bone tissue engineering application is the biodegradable polymer poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). PHBV, a microbial polymer, has gained special importance because of favorable mechanical characteristics, biological properties, highly adaptable structure, non-toxic degradation products, and minimal inflammatory response when used as scaffold. However, the lack of natural cell recognition sites on PHBV has resulted in delayed cell attachment, proliferation, differentiation, and tissue regeneration on the polymer. The primary objective of this research is to modify PHBV so as to improve the biocompatibility of the matrix. An approach was developed to prepare porous and bioactive molecule coated scaffold so as to improve the biocompatibility of PHBV matrix.

ISBN: 9780549883142Subjects--Topical Terms:

1018428
Chemistry, Polymer.

Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications.
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020 $a 9780549883142
035 $a (UMI)AAI3335311
035 $a AAI3335311
040 $a UMI $c UMI
100 1 $a Liu, Hui. $3 803598
245 1 0 $a Tailoring of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers for bone tissue applications.
300 $a 118 p.
500 $a Adviser: D. Raghavan.
500 $a Source: Dissertation Abstracts International, Volume: 69-11, Section: B, page: 6832.
502 $a Thesis (Ph.D.)--Howard University, 2008.
520 $a Considerable scientific and technological progress has been made in formulating biomaterials based on natural and synthetic polymers for ultimate use in bone tissue scaffolding. One class of polymers that has drawn attention in recent years for bone tissue engineering application is the biodegradable polymer poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). PHBV, a microbial polymer, has gained special importance because of favorable mechanical characteristics, biological properties, highly adaptable structure, non-toxic degradation products, and minimal inflammatory response when used as scaffold. However, the lack of natural cell recognition sites on PHBV has resulted in delayed cell attachment, proliferation, differentiation, and tissue regeneration on the polymer. The primary objective of this research is to modify PHBV so as to improve the biocompatibility of the matrix. An approach was developed to prepare porous and bioactive molecule coated scaffold so as to improve the biocompatibility of PHBV matrix.
520 $a We investigated the role of porosity, collagen coating, and ozone treatment of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) scaffold on cell proliferation. Based on biochemical assay, we established that maximum cell proliferation occurs on collagen coated and ozone treated porous PHBV film followed by collagen coated porous PHBV film than on porous PHBV film and the least on nonporous PHBV film. Confocal microscopy in combination with biochemical assay was used to generate 3D map of viable cell proliferation on the bulk PHBV matrix. The cells were cultivated on modified PHBV film in mineralization media containing beta-glycerol phosphate for predetermined time interval and later calcium deposits were stained with alizarin red-S assay. Atomic absorption (AA) technique was used to measure the Ca2+ content of the medium and it was found that the longer the cells were incubated in organic phosphate medium, the greater amount of Ca2+ from the medium is mineralized.
520 $a The phenotypic character of the cells grown on modified PHBV matrix was compared against TCPS (a positive control). RNA was extracted from the UMR-106 cells, and reverse transcriptase-polymerase chain reaction (RT-PCR) was applied to detect expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (a house keeping gene) and bone sialoprotein (BSP) (marker of the osteoblastic phenotype) on both matrices. Cells grown on both modified porous and nonporous PHBV were found to retain their original phenotypic character.
520 $a In addition to evaluating the biocompatibility of modified PHBV, an attempt was made to investigate the degradability of PHBV in physiological medium (phosphate buffer medium at 37°C). The degradation was followed by monitoring the time dependent changes in chemical composition and mass loss of the macroporous films. FT-1R data of the bulk film showed ester hydrolysis upon long immersion of PHBV film in buffer medium. The mass loss of PHBV film after 19 weeks of exposure to buffer medium was found to range from 2.8 % to 9.2 % with a strong dependence on the HV content of the original copolyester. Like mass loss data, the NMR results showed a big drop in the mol% of HV content for degraded copolymer with high HV content of the copolymer. Information generated from this study can be useful in the selection of appropriate PHBV copolymer for clinical applications where the biopolymer needs to remain intact during the period of short term use.
590 $a School code: 0088.
650 4 $a Chemistry, Polymer. $3 1018428
650 4 $a Engineering, Materials Science. $3 1017759
690 $a 0495
690 $a 0794
710 2 $a Howard University. $3 1017554
773 0 $t Dissertation Abstracts International $g 69-11B.
790 $a 0088
790 1 0 $a Raghavan, D., $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3335311