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Evaluation and Determination of the ...

Macharia, John Macharia,

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Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management =/ John Macharia Macharia.
其他題名:	Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
作者:	Macharia, John Macharia,
面頁冊數:	1 electronic resource (150 pages)
附註:	Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
Contained By:	Dissertations Abstracts International86-03B.
標題:	Fatalities. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31476312
ISBN:	9798384327233

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
Macharia, John Macharia,

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management =Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /John Macharia Macharia. - 1 electronic resource (150 pages)

Source: Dissertations Abstracts International, Volume: 86-03, Section: B.

Colorectal cancer is among the most common malignancies and the third prime cause of cancer-associated mortalities. Research into tumorigenic pathways can aid in the development of more efficient cancer therapies and provide insight into the physiological regulatory mechanisms employed by rapidly proliferating cancer cells. Due to the severe side effects of cancer chemotherapeutic medications, plant metabolites, and their analogs are now explored more frequently for the treatment and prevention of colorectal cancer (CRC), opening the stage for new phytotherapeutic strategies that are considered effective and safe substitutes. This extensive research aimed to evaluate and determine the phytotherapeutic properties of selected plants and their bioactive metabolites on targeted genes in colorectal cancer management. The plants evaluated were Withania somnifera L, Warbugia ugandensis, and Aloe secundiflora, while the targeted genes were COX-2, CASP9, Bcl-xL, Bcl2 and 5-LOX in CRC cell lines (Caco-2). To establish critical gaps in this important topic, extensive and well-thought systematic reviews were conducted at the initial stage of the study. Results obtained from the reviews informed the choice of target genes for selective consideration. Plant extracts were obtained using serial exhaustive extraction using three different extraction solvents with varying polarities, for maximum metabolites extraction (Hexane, Ethyl acetate, and Methanol). They were then dissolved in Dimethyl sulfoxide (DMSO) appropriately for bioassay. Caco-2 cell lines were passaged, and treated with plant extracts at varying concentrations, and their RNA's isolated for evaluation. Our unique study reports on these important plants as efficient natural inhibitors of CRC growth, by directly linking their phytoconstituents to; the downregulation of COX-2, 5-LOX, Bcl-xL, Bel2, and upregulation of CASP9 genes dose-dependently. I strongly present Withania somnifera, Warbugia ugandensis, and Aloe secundiflora plant extracts as promising natural inhibitors for CRC carcinogenesis and recommend in vivoand subsequent clinical trials, with substantial clinical effects postulated. In addition, methanol and ethyl acetate can extract metabolites exhibiting higher regulatory effects on the targeted genes and are thus highly recommended for application in yielding maximum benefits. Finally, I suggest studies on the identification and characterization of the specific metabolites in these plants, that are involved in the modulatory mechanisms, resulting in the inhibition of CRC growth and possible metastases.

English

ISBN: 9798384327233Subjects--Topical Terms:

3681792
Fatalities.

Evaluation and Determination of the Phytotherapeutic Properties of Selected Plants and Their Bioactive Metabolites on Targeted Genes in Colorectal Cancer Management = = Egyes Gyogynovenyek es Bioaktiv Metabolitjaik Fitoterapias Tulajndonsagainak Ertekelese es Meghatarozasa a Vastagbelrakban Szerepet Jatszo Genek Tekinteteben /
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