東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁 到查詢結果 [ null ]

切換: 標籤 | MARC模式 | ISBD

Advancements and Challenges in Cigua...

Prytulka, Natasha,

FindBook

Google Book

Amazon

博客來

Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B // Natasha Prytulka.
作者:	Prytulka, Natasha,
面頁冊數:	1 electronic resource (106 pages)
附註:	Source: Masters Abstracts International, Volume: 85-11.
Contained By:	Masters Abstracts International85-11.
標題:	Environmental science. -
電子資源:	https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31295263
ISBN:	9798382563879

Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B /
Prytulka, Natasha,

Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B /Natasha Prytulka. - 1 electronic resource (106 pages)

Source: Masters Abstracts International, Volume: 85-11.

The detection of ciguatoxins (CTXs) in biological samples is challenging due to their low concentrations, the presence of various congeners, and the absence of standardized methods. This study uses high resolution mass spectrometry (HRMS) with P-CTX-3B as a reference standard. The protonated molecules ([M+H]+) were most prevalent, especially when acetonitrile/water was utilized, providing enhanced sensitivity. Optimized collision energies of 15 eV for protonated molecules and flow rates of 10 µl/min enhance sensitivity and peak intensities, respectively. Acetonitrile/water (ACN/H2O) is recommended as the primary solvent for HRMS method, an aspect underexplored in existing literature. The detection of CTX-3B in fish tissue samples proved to be challenging, caused by variations in ion peak intensities and matrix effects, requiring a deeper exploration of the impact of complex matrices on CTX detection. The study emphasizes the need for a reliable internal standard to mitigate these effects and highlights the ongoing challenge of developing a rapid, simple, and sensitive detection method. The study's specific focus on the P-CTX-3B analogue significantly contributes to methodology development for this congener, serving as a foundational step in understanding and detecting CTX. Despite notable progress, the study acknowledges the absence of an ideal assay, outlining key challenges for future research on ciguatera analysis. It underscores the continuous necessity for method reevaluation, testing, and the broader goal of establishing a more clarified and rugged method for the identification of CTX in fish.

English

ISBN: 9798382563879Subjects--Topical Terms:

677245
Environmental science.
Subjects--Index Terms:

Analytical chemistry

Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B /
LDR:03217nmm a22004693i 4500 001 2437350
005 20251119142759.5
006 m o d
007 cr|nu||||||||
008 261011s2024 miu||||||m |||||||eng d
020 $a 9798382563879
035 $a (MiAaPQD)AAI31295263
035 $a AAI31295263
040 $a MiAaPQD $b eng $c MiAaPQD $e rda
100 1 $a Prytulka, Natasha, $e author. $3 3828297
245 1 0 $a Advancements and Challenges in Ciguatoxin Detection: Developing a High-Resolution Mass Spectrometric Method for the Identification of P-CTX-3B / $c Natasha Prytulka.
264 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2024
300 $a 1 electronic resource (106 pages)
336 $a text $b txt $2 rdacontent
337 $a computer $b c $2 rdamedia
338 $a online resource $b cr $2 rdacarrier
500 $a Source: Masters Abstracts International, Volume: 85-11.
500 $a Advisors: Hintelmann, Holger; Stock, Naomi Committee members: Metcalfe, Chris.
502 $b M.S. $c Trent University (Canada) $d 2024.
520 $a The detection of ciguatoxins (CTXs) in biological samples is challenging due to their low concentrations, the presence of various congeners, and the absence of standardized methods. This study uses high resolution mass spectrometry (HRMS) with P-CTX-3B as a reference standard. The protonated molecules ([M+H]+) were most prevalent, especially when acetonitrile/water was utilized, providing enhanced sensitivity. Optimized collision energies of 15 eV for protonated molecules and flow rates of 10 µl/min enhance sensitivity and peak intensities, respectively. Acetonitrile/water (ACN/H2O) is recommended as the primary solvent for HRMS method, an aspect underexplored in existing literature. The detection of CTX-3B in fish tissue samples proved to be challenging, caused by variations in ion peak intensities and matrix effects, requiring a deeper exploration of the impact of complex matrices on CTX detection. The study emphasizes the need for a reliable internal standard to mitigate these effects and highlights the ongoing challenge of developing a rapid, simple, and sensitive detection method. The study's specific focus on the P-CTX-3B analogue significantly contributes to methodology development for this congener, serving as a foundational step in understanding and detecting CTX. Despite notable progress, the study acknowledges the absence of an ideal assay, outlining key challenges for future research on ciguatera analysis. It underscores the continuous necessity for method reevaluation, testing, and the broader goal of establishing a more clarified and rugged method for the identification of CTX in fish.
546 $a English
590 $a School code: 0513
650 4 $a Environmental science. $3 677245
650 4 $a Chemistry. $3 516420
650 4 $a Toxicology. $3 556884
650 4 $a Food science. $3 3173303
653 $a Analytical chemistry
653 $a Ciguatera fish poisoning
653 $a Ciguatoxin
653 $a High-resolution mass spectrometry
653 $a Optimization
653 $a P-CTX-3B
690 $a 0768
690 $a 0485
690 $a 0383
690 $a 0359
710 2 $a Trent University (Canada). $b Environmental and Life Sciences. $e degree granting institution. $3 3770473
720 1 $a Hintelmann, Holger $e degree supervisor.
720 1 $a Stock, Naomi $e degree supervisor.
773 0 $t Masters Abstracts International $g 85-11.
790 $a 0513
791 $a M.S.
792 $a 2024
856 4 0 $u https://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=31295263